胃癌相关基因表达与其生物学行为关系研究

目的探讨P21、细胞周期素D1(CyclinD1)及P53基因表达与胃癌生物学行为的关系。方法应用原位杂交免疫组化技术检测74例胃癌组织及癌旁正常粘膜P21和CyclinD1mRNA及P53蛋白表达,分析三者与胃癌生物学行为的关系。结果74例胃癌组织和癌旁正常粘膜中,P21、CyclinD1及P53表达阳性率分别为43.2%和55.4%、40.5%和78.4%、25.7%和1.4%,三者比较均有显著性差异(P<0.01)。P21、CyclinD1及P53表达与患者性别、年龄、分期无明显相关性(P>0.05),与淋巴结转移显著相关(P<0.05);胃癌分化程度与P21阳性表达呈正相关,与CyclinD1阳性表达呈负相关。结论胃癌中P21低表达、CyclinD1和P53过表达可能促使胃癌细胞增殖,且具有更强的侵袭力;多基因表达异常可作为评价胃癌预后的重要参考指标。

Relationship between the biological behavior of gastric carcinoma and the expression P21,CyclinD1 and P53 genes

Objective To investigate the correlation between the biological behavior of gastric carcinoma and the expression of P~21 ,CyclinD_1 and P~53 genes.Methods Hybridyzation in situ and immunohistochemical techniques were used to detect the expression of P~21 mRNA,CyclinD_1mRNA and P~53 proteins of gastric carcinoma.Results The positive rates of P~21 ,CyclinD_1 and P~53 in 74 cases of gastric carcinoma and normal mucose were 43.2%,55.4%,40.5% and 78.4%,25.7%,1.4% respectively and was significantly different (P<0.01).There was no relationship between the expression of P~21 ,CyclinD_1 and P~53 proteins in gastric carcinoma and patient age ,sex,stage(P>0.05).There was relationship between the expression of P~21 ,CyclinD_1 and P~53 proteins and lymph node metastasis (P<0.05).The positive rates of P~21 expression and the degrees of differentiation in gastric carcinoma were postively correlated.The positive rates of CyclinD_1 expression and the degrees of differentiation in gastric carcinoma were negatively correlated.Conclusion The lose of P~21 expression and the over-expression of CyclinD_1 and P~53 may contribute to the proliferation of gastric cancer cell ,making them more aggressive ,which may serve as important indicator of prognosis of gastric carcinoma.