Nucleostemin基因在姜黄素抑制胃癌SGC-7901细胞增殖中的意义

目的探讨NS基因在姜黄素抑制胃癌SGC-7901细胞株增殖和诱导凋亡中的作用。方法不同浓度姜黄素作用于胃癌SGC-7901细胞株,利用MTT法检测细胞增殖抑制率,RT-PCR法检测姜黄素作用前后NS基因表达量的变化,流式细胞仪法检测细胞周期和细胞凋亡情况。结果姜黄素能显著抑制体外培养的胃癌SGC-7901细胞株增殖,并呈量效和时效关系;流式细胞术显示G0/G1期细胞增加,S期细胞减少,检出亚二倍体凋亡峰;RT-PCR显示NS基因表达量下降。结论姜黄素显著抑制胃癌SGC-7901细胞株增殖,并促进凋亡,其发生可能与NS基因表达下降有关。

Significant of NS gene on curcumin inhibitory cell proliferation in gastric carcinoma SGC-7901cells

Objective ： To investigate the effect of NS gene on gastric - carcinoma cells proliferation and apoptosis induced by cur- 3 cumin . Methods： SGC -7901cells were treated with various of curcumin , we detected the rate of cell proliferation inhibition by MTT assay; detected the levels of NS gene expression in all groups by RT - PCR; detected cell cycle and cell apoptosis by flow cytometry. Results： Curcumin could effectively inhibit the SGC -7901cells proliferation in dose -dependent and time -dependent manner , reduce the level of NS gene expression , the percent of G0/G1 phase cell increase with the decrease of S phase and sub - peak of apoptosis appear. Conclusion： Curcumin could substantially inhibit SGC -7901 cells proliferation , inducing apoptosis probably through down regulating NS gene expression.