抗内皮细胞抗体的检测及临床应用

目的 建立可以常规应用的免疫荧光法检测血清中抗内皮细胞抗体（AECA）。方法 对试验条件进行研究并与ELISA法对比，检测冠心病、脑梗死、肺疾患及脑血管障碍患者310例，青年健康组50例，老年健康组30例。结果 两法检测阳性率无显著差异（P＞0．05），符合率89．7％；试验表明，用两种方法检测冠心病组、脑梗死组的AECA阳性率均明显高于健康组（P＜0．01），肺疾患组与脑血管障碍组也高于健康组（P＜0．05），内皮素检测证实AECA阳性者与内皮素升高明显正相关。结论 自身免疫形成的AECA在促进内皮细胞损伤中具有重要意义；免疫荧光法快速、简便，适用于临床应用。

Determination of Anti-endotheliocyte Antibody and Its Application

Objective To establish a routinely used immuno-fluorescence method for detecting anti-endotheliocyte antibody (AECA).Methods Optimized the test condition and compared with ELISA of AECA in 4 patient's groups(coronary heart disease,cerebro-infarction,pneumonic disease,and cerebro-vascular disease),young and old healthy groups.Results The positive rate of two methods showed no significant difference ( P >0.05),the agreement rate was 89.7%.The AECA positive rate of two methods were all significant higher in diseased groups than that in healthy groups ( P <0.01 or P <0.05).The endothelion in AECA positive sera were correlated higher.Conclusion AECA plays an important role in promoting endothelicyte lesion.Immuno-fluoresence method was simple,rapid and applicable.