miR-146a在食管鳞癌中的表达及意义

目的： 分析食管鳞癌(esophageal squamous cell carcinoma, ESCC)组织中miR-146a的表达情况及其与食管鳞癌临床病理特征之间的关系。方法： 采用茎环结构的逆转录实时定量PCR方法检测50例ESCC患者癌组织及癌旁正常组织中miR-146a的表达情况，并分析其与临床病理特征间的关系。结果： miR 146a在癌组织中的表达显著高于癌旁正常组织（Z=-3.162；P<0.01）；miR-146a的高表达分别与食管癌的分化程度、淋巴结转移、TNM分期显著相关（P<0.01）。结论： miR-146a的高表达在ESCC的发生发展中发挥重要作用。

Expression and clinical significance of miR-146a in human esophageal squamous cell carcinoma

Objective: To investigate the expression of miR-146a in esophageal squamous cell carcinoma(ESCC) and analyze its correlation with clinico-pathological features of ESCC.Methods: Stem-loop RT-PCR was used to detect the expression of miR-146a in 50 ESCC tissues and matched tumor-free tissues,and to analyze its correlation with clinico-pathological features of ESCC.Results: The expression of miR-146a was significantly increased in ESCC tissues compared with the matched tumor-free tissues(Z=-3.162,P<0.01).Up-regulation of miR-146a expression was correlated with differentiation degree,lymph node metastasis and clinical stage in ESCC(P<0.01).Conclusion: MiR-146a in ESCC tissues was significantly higher than that in matched tumor-free tissues,which may play a significant role in the occurrence and development of ESCC.