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| 不同固定剂及不同浓度对细菌保存的影响 | |
| 引用本文： | 滕飞鹏,付春英,喻奇燕,陈兴艳. 不同固定剂及不同浓度对细菌保存的影响[J]. 检验医学与临床, 2009, 6(2): 96-97 |
| 作者姓名： | 滕飞鹏 付春英 喻奇燕 陈兴艳 |
| 作者单位： | 1. 四川省人民医院检验科,成都,610072 2. 遵义医学院 3. 四川省人民医院小儿科 |
| 摘 要： | 目的探讨固定剂1（A）、固定剂2（B）、固定剂3（C）、防腐剂1（D）和防腐剂2（E）不同种类的固定剂和防腐剂及不同固定浓度对细菌保存的影响。方法采用正交试验安排试验以变异系数为指标进行固定剂混悬液最优配方的筛选。结果优化的固定剂配方最优组合为A183C1D1E1。结论不同的固定剂在不同的固定时间对细菌的保存有显著的影响。优化的固定剂配方对细菌有良好的保存作用，该技术对于尿沉渣细菌的质控物的研制有重要应用价值。 |
| 关 键 词： | 固定剂 细菌 尿液分析 |
Effects of different fixed agents and concentrations on bacteria preservation |
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| TENG Fei-peng,FU Chun-ying,YU Qi-yan,CHEN Xing-yan. Effects of different fixed agents and concentrations on bacteria preservation[J]. Laboratory Medicine and Clinic, 2009, 6(2): 96-97 | |
| Authors: | TENG Fei-peng FU Chun-ying YU Qi-yan CHEN Xing-yan |
| Affiliation: | TENG Fei-peng1,FU Chun-ying1,YU Qi-yan2,CHEN Xing-yan3.1.Department of Clinical Laboratory,Sichuan Provincial People's Hospital,Chengdu 610072,China,2.Zunyi Medical College,Zunyi 563000,3.Department of Peadiatrics |
| Abstract: | Objective To investigate the effects of different fixed agents(aldehydes,spirits,thymol,and preservatives)and concentrations on bacteria preservation.Methods Optimal compounding of fixative suspension was determined with the orthogonal design and variation coefficient.Results The optimal composition of fixatives was A1B3C1D1E1.Conclusion Bacteria's conservation is markedly affected by different kinds of fixatives and different fixation time.The optimization of fixatives' reagent is good for bacteria's conse... |
| Keywords: | fixatives bacteria urine analysis |
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