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| 白介素-6拮抗N-甲基-D-天门冬氨酸诱导的神经元凋亡 | |
| 引用本文： | 刘展,黄慧伟,黄彦,彭聿平. 白介素-6拮抗N-甲基-D-天门冬氨酸诱导的神经元凋亡[J]. 南通医学院学报, 2009, 29(2) |
| 作者姓名： | 刘展 黄慧伟 黄彦 彭聿平 |
| 作者单位： | 南通大学医学院生理学教研室,南通,226001 |
| 基金项目： | 国家自然科学基金面上项目，江苏省高等学校优秀科技创新团队项目，江苏省卫生厅科研项目，南通市应用研究计划 |
| 摘 要： | 目的:在抗凋亡成分Bcl-2、促凋亡成分Bax和凋亡关键酶半胱氨酸蛋白酶-3(cysteinyl asparate-specific proteinase-3,caspase-3)的基因水平,探讨IL-6保护神经元防止N-甲基-D-天门冬氨酸(NMDA)诱导的神经元凋亡。方法:取出生后8d幼鼠的小脑进行颗粒神经元体外培养。在培养液中加入IL-6(40ng/ml),孵育8d后,用NMDA(100μmol/L)刺激小脑颗粒神经元30min,诱导神经元凋亡。用Real-timePCR法检测神经元内Bcl-2、Bax和caspase-3的mRNA表达水平。结果:未用IL-6预处理的神经元,在用NMDA刺激后,其表达Bcl-2mRNA显著减少,表达BaxmRNA及caspase-3mRNA明显增加。IL-6本身没有明显影响上述基因的表达。神经元用IL-6预孵育后,再用NMDA刺激,神经元内Bcl-2、Bax和caspase-3的mRNA表达水平与未用IL-6预处理的神经元比较,表现为Bcl-2上调、Bax和caspase-3下调的变化,这些变化均有显著意义。结论:NMDA可诱导神经元的凋亡,IL-6对NMDA诱导的神经... |
| 关 键 词： | 神经元凋亡 白介素-6 N-甲基-D-天门冬氨酸 Bcl-2 Bax 半胱氨酸蛋白酶-3 大鼠 |
Interleukin-6 prevents NMDA-induced apoptosis of the cultured cerebellar granule neurons |
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| LIU Zhan,HUANG Huiwei,HUANG Yan,et al. Interleukin-6 prevents NMDA-induced apoptosis of the cultured cerebellar granule neurons[J]. ACTA Academiae Medicinae Nantong, 2009, 29(2) | |
| Authors: | LIU Zhan HUANG Huiwei HUANG Yan et al |
| Affiliation: | Department of Physiology;School of Medicine;Nantong University;Nantong 226001 |
| Abstract: | Objective: We explored that IL-6 protected neurons against NMDA-induced apoptosis at the gene levels of anti- apoptotic Bcl-2, pro-apoptotic Bax and apoptotic enzyme cysteinyl asparate-specific proteinase-3 (caspase-3). Methods: The cerebellar granule neurons from postnatal 8-day infant rats were chronically exposed to IL-6 (40 ng/ml), NMDA (100 μmol/L) was then added to the cultures and stimulated the neurons for 30 min to induce the neuronal apoptosis. Real- time PCR was employed to detect the expression ... |
| Keywords: | Bcl-2 Bax |
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