| EB病毒转化B淋巴细胞建立的永生细胞株长期传代遗传稳定性的研究 |
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| 引用本文： | 李彦涵,黄小琴,林克勤,陶玉芬,易文,姚宇峰,史磊,褚嘉祐.EB病毒转化B淋巴细胞建立的永生细胞株长期传代遗传稳定性的研究[J].中华医学遗传学杂志,2008,25(3). |
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| 作者姓名： | 李彦涵 黄小琴 林克勤 陶玉芬 易文 姚宇峰 史磊 褚嘉祐 |
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| 作者单位： | 中国医学科学院北京协和医学院医学生物学研究所遗传室,昆明,650118 |
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| 基金项目： | 美国中华医学会资助项目 |
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| 摘 要： | 目的 研究EB病毒转化外周血B淋巴细胞建成的永生细胞株在传代过程中遗传特性是否发生改变.方法 对10株永生细胞株进行30代的长期传代培养,采用染色体显带、微卫星DNA及端粒酶活性检测技术,观察不同传代次数的永生细胞株二倍性、染色体核型、微卫星DNA及端粒酶活性的改变.结果 永生细胞株在30代以内,在染色体二倍性、核型、微卫星DNA方面保持稳定,未检测出端粒酶活性.结论 经EB病毒转化的永生细胞株在有限代次内遗传特征是稳定的.
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| 关 键 词： | 永生细胞株 染色体二倍性 微卫星DNA 端粒酶活性 遗传稳定性 |
Study on the genetic stability of immortalized cell line of lymphocyte cell transformed by EB virus afar long subculture process |
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| LI Yan-han,HUANG Xiao-qin,LIN Ke-qin,TAO Yu-fen,YI Wen,YAO Yu-feng,SHI Lei,CHU Jia-you.Study on the genetic stability of immortalized cell line of lymphocyte cell transformed by EB virus afar long subculture process[J].Chinese Journal of Medical Genetics,2008,25(3). |
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| Authors: | LI Yan-han HUANG Xiao-qin LIN Ke-qin TAO Yu-fen YI Wen YAO Yu-feng SHI Lei CHU Jia-you |
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| Abstract: | Objective To study the genetic stability of an immortalized cell line transformed by Epstein-Barr virus(EBV)after long subculture process.Methods In the present study,the genetic stabihty including chromosome diploidy,karyotypes and microsatellite DNA were evaluated with chrolnosome banding techniques and microsatellite DNA detection.The telomerase activity of the immortalized cell line was detected by using the telomerase assay kit.Results From passage 1 to 30,there were no change of the diploidy,karyotypes of chiomosome mad micmsatellite DNA,and the telomerase activity is negative.Condusion This study indicates that the immortalized cell line remains stable genefically within limited passages. |
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| Keywords: | immortalized cell line chromosome diploiay mierosatellite DNA telomerase activity genefie stability |
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