乙肝病毒基因组中U5序列在乙肝患者垂直传递中的分析研究

目的研究乙肝病毒在乙肝患者垂直传递中的情况，方法运用分子遗传学的技术方法：单核苷酸多态性（SNP）及多聚酶链反应-单链构相多态性（PCR-SSCP），对30个家庭（68个病例）的乙肝患者及其子女进行了研究。结果在乙肝患者及其受感染后出生子女中游离型及整合型乙肝病毒持续增高，与感染前所生子女间比较差异显著（P〈0．05），SNP分析结果显示在乙肝病毒基因组中，U5序列和非U5序列中许多基因位点发生了碱基替换，插入或缺失，男性乙肝患者及其受感染子女的SNP分析结果被确定。结论乙肝病毒可通过男性患者传递至子女；至此，又一个分子遗传学证据证实了了乙肝病毒的遗传性传递。

A research of HBV genome U5 sequence in hepatitis B patients and their children

Objective: To study vertical transmission of hepatitis B between the hepatitis B patients and their children. Methods: 30 families (68 cases) of hepatitis B patients and their children were examined by the molecular genetic methods and techniques of single nucleotide polymorphism(SNP) and polymerase chain reaction -single strand configuration polymorphism( PCR- SSCP). Results: The frequencies of freed and integrated HBV DNA were higher consistently between the hepatitis B patients and their children born after the infection of hepatitis B, and the difference was more significance than the children born before their parent suffered from hepatitis B, ( P ＜ 0.05 ). SNP of hepatitis B patients and their children was ananlysed and it was discovered that there were base substitution, insertion or deletion in the many base site of HBV genome U5 sequence and non - U5 sequence; the SNP of male hepatitis B patients and their children born after the occurrence of hepatitis B was identical. Conclusion: Hepatitis B may be inherited from the male hepatitis B patients to their children born after the occurrence of hepatitis B; thereby, another molecular genetic evidence is provided for hereditary transmission of hepatitis B.