胃癌组织脆性组氨酸三联体基因杂合性丢失的临床意义

目的检测脆性组氨酸三联体(FHIT)基因在胃癌(GC)、不典型增生(DP)和肠上皮化生(IM)组织中的杂合性丢失(loss of heterozygosity,LOH),研究LOH在GC发生中的作用。方法采用PCR方法检测GC、DP和IM组织和正常对照胃黏膜组织中FHIT基因多态性位点D3S1234和D3S1300中的LOH。结果 GC组、DP组和IM组在D3S1234位点的LOH发生率分别为33.0%,29.0%和11.0%;在D3S1300位点的LOH发生率分别为34.0%,33.0%和8.0%。GC组和DP组在D3S1234和D3S1300位点的LOH发生率显著高于IM组,GC组与DP组间差异无统计学意义。结论 FHIT基因的LOH可能是GC形成过程中的早期事件,能用于GC高危人群筛查和早期诊断的研究。

Clinical significance of loss of heterozygosity of FHIT gene in tissues of gastric cancer

Objective To study the loss of heterozygosity（LOH） of FHIT gene in tissues of gastric cancer（GC）,dysplasia（DP）and intestinal metaplasia（IM）,and to reveal the effects of the LOH of FHIT gene in the carcinogenesis of GC.Methods The LOH at microsatellites loci D3S1234 and D3S1300 of FHIT gene was measured in 100 GC tissues,100 DP tisues and 100 IM tissues and their corresponding 100 normal gastric mucosal tissues by PCR.Results The LOH at D3S1234 locus was detected positive in 33.0% of GC tissues,29.0%of the DP tissues and 11.1% of the IM tissues;and the LOH at D3S1300 locus was detected positive in 34.0% of the GC tissues,33.0%of the DP tissues and 8.0% of the IM tissues respectively.The LOH rates at D3S1234 and D3S1300 loci of the GC and atypical hyperplasia tissues were higher than that of the IM tissues.No significant differences of the LOH rates at D3S1234 and D3S1300 loci were found between the GC and DP mucosal tissues.Conclusion The loss of heterozygosity of FHIT gene may be an early event in the tumorigenesis of GC,which may has roles in the screening and early diagnosis of GC.