严重自发空泡化对胚胎发育潜能的影响

目的:探讨授精后第5天(D5)严重自发空泡化的形成对胚胎发育潜能的影响。方法:回顾性分析2016年1月-2017年12月于本中心行体外受精-胚胎移植(IVF-ET)或卵细胞胞质内单精子注射术(ICSI)治疗患者的临床资料,选取授精后D5出现严重自发空泡化的873个胚胎作为空泡组,同期形态无异常的5709个胚胎作为对照组。比较两组第6天(D6)胚胎的可利用囊胚率、优质囊胚率及临床结局。结果:空泡组D6可利用囊胚率(8.3%)及优质囊胚率(22.2%)均低于对照组(27.9%、65.2%)(P<0.05)。临床结局方面,无论是单胚复苏移植还是与正常胚胎的联合复苏移植,空泡组与对照组的妊娠率、活产率和流产率均无差异(P>0.05)。结论:授精后D5严重自发空泡化的形成虽然影响D6可利用囊胚率及优质囊胚率,但可利用囊胚与对照组有相似的临床结局。

The influence of severe spontaneous vacuolation on the potential of embryonic development after insemination

Objective:To investigate the influence of severe spontaneous vacuolation on the potential of embryonic development on the 5th day(D5)after insemination.Methods:The clinical data of the women experienced in vitro fertilization and embryo transfer(IVF-ET)or intracytoplasmic sperm injection(ICSI)from January 2016 to December 2017 were retrospective analyzed.873 embryos with severe spontaneous vacuolation on the D5 after insemination were selected in study group,and 5709 embryos without abnormal morphology were randomly selected in control group.The blastocyst rate,high quality blastocyst rate,and clinical outcomes of women on the 6th day(D6)after insemination were compared between the two groups.Results:The blastocyst rate and high quality blastocyst rate of women in the study group were 8.3%and 22.2%,which were significant lower than those(27.9%and 65.2%)of women in the control group(P<0.05).In terms of clinical outcomes,there were no significant different in pregnancy rate and live birth rate of women after embryo transfer with single embryo resuscitation or combined resuscitation with normal embryos between the two groups(P>0.05).Conclusion:Although the formation of severe spontaneous vacuolation on the D5 after insemination can affect the blastocyst available rate and high-quality blastocyst rate on the D6,the blastocyst available rate of women with the formation of severe spontaneous vacuolation on the D5 after insemination has the similar clinical outcomes of women without abnormal morphology of embryos.