胚胎发育时期接触过乙酸羟孕酮的成年雄性大鼠的肝脏代谢改变(英文)

目的:观察胚胎在子宫内接触乙酸羟孕酮对雄性大鼠成年后肝脏代谢的影响。方法:让怀孕1天、7天和14天的 Wistar 白化大鼠分别接触高于正常浓度的乙酸羟孕酮(10 mg/kg 和25 mg/kg)。出生的雄性仔鼠在可控条件下喂养,出生90天后处死,迅速切取肝脏组织称重,并用于生化分析。结果:胚胎发育时期接触过乙酸羟孕酮的大鼠肝脏中的琥珀酸酯脱氢酶、谷氨酸酯脱氢酶、葡萄糖-6-磷酸盐脱氢酶、苹果酸酯脱氢酶以及转氨酶(aminotransaminases)的活性显著提高,乳酸脱氢酶的活性显著下降。此外,与对照组相比,随着实验大鼠肝脏组织脂质氧化反应的增加,抗氧化酶(谷胱甘肽 S 型转移酶和过氧化氢酶)活性也有显著增加。结论:上述实验结果表明胚胎发育时期接触过乙酸羟孕酮的大鼠的氧化代谢、抗氧化机制以及脂质过氧化水平都得到了提高。转氨酶活性的增加表明线粒体的完整性被损伤和破坏。

Alterations in hepatic metabolism of adult male rats following exposure to hydroxyprogesterone during embryonic development

Aim:To investigate the effect of in utero exposure to hydroxyprogesterone(HP)on liver metabolism in adult male albino rats.Methods:Pregnant Wistar strain albino rats were exposed to supra-normal levels(10 mg/kg and 25 mg/kg)of HP on days 1,7 and 14 of pregnancy.The male pups were maintained under controlled conditions and the rats were killed 90 days after birth.The liver tissue was immediately excised,weighed and used for biochemical assays. Results:The activity levels of succinate dehydrogenase(SDH),glutamate dehydrogenase(GDH),glucose-6-phosphate dehydrogenase(G-6-PDH),malate dehydrogenase(MDH)and aminotransaminases were significantly increased in the livers of rats exposed to HP during embryonic development.The lactate dehydrogenase(LDH)activity level was significantly decreased in the liver of experimental rats.Furthermore,there was a significant elevation of activity levels of antioxidant enzymes(glutathione S-transferase[GST] and catalase[CAT])with an increased lipid peroxidation in the hepatic tissue of experimental rats compared with the control group.Conclusion:The results of the present study suggest that there is an increase in the oxidative metabolism,antioxidative mechanism and levels of lipid peroxidation in rats exposed to HP during embryonic development.The increased aminotransaminase activities in these rats reveal tissue damage and disruption of mitochondrial integrity.(Asian J Androl 2006 Jul;8:463-467)