督脉电针对大鼠脊髓损伤后神经功能恢复的影响

目的探讨督脉电针治疗对大鼠脊髓损伤后神经功能恢复的影响。方法利用多中心急性脊髓损伤打击器建立大鼠T11脊髓损伤模型,建模成功后随机分为对照组(A组)和督脉电针组(B组)。脊髓损伤后1周,B组接受督脉电针治疗。两组大鼠于脊髓损伤后1、2、4、8周行BBB后肢运动功能评分,脊髓损伤后2、4、8周行体感诱发电位(SEP)检测,脊髓组织行HE染色与神经丝蛋白(NF200)免疫组化染色。结果 SCI后1周,两组大鼠BBB后肢运动功能评分无显著性差异(P>0.05);SCI后2、4、8周,B组BBB后肢运动功能评分较A组明显升高(P<0.01),SEP潜伏期明显缩短、波幅明显增高(P<0.01)。HE染色显示损伤区瘢痕组织及空洞形成,B组脊髓空洞比A组小;脊髓组织NF200阳性表达B组各时间点较A组明显增强(P<0.01)。结论督脉电针治疗可有效促进SCI大鼠神经功能恢复。

Effects of Du Meridian Electroacupuncture on Neurological Function of Rats after Spinal Cord Injury

Objective To investigate the effect of Du meridian electroacupuncture on the neurological function of rats after spinal cord injury(SCI).Methods The SCI model of rats was induced with MASCIS Impactor at the level of T11 segment and were equally divided into control group(group A) and Du meridian electroacupuncture group(group B).Group B received electroacupuncture once a day 1 week after SCI.They were assessed with Basso-Beattie-Bresnahan(BBB) score 1,2,4,8 weeks after SCI,somatosensory evoked potential(SEP) and spinal cord histopathological examination 2,4,8 weeks after SCI.Results The difference of BBB score was not significant between the groups 1 week after SCI(P>0.05),but improved more in group B than in group A 2,4,8 weeks after SCI(P<0.01),as well as the latency and wave amplitude of SEP(P<0.01).There were scar tissue and syringomyelia in injured spinal cord,and the size of syringomyelia was larger in group A than in group B.Neurofilament 200(NF200) expressed 2,4,8 weeks after SCI,and was more in group B than in group A(P< 0.01).Conclusion Du meridian electroacupuncture can promote the recovery of neurological function in rats after spinal cord injury.