类风湿关节炎患者外周血白细胞介素-22的表达及临床意义

目的 测定类风湿关节炎(RA)患者血清中IL-22的水平并分析其临床意义。方法 采用ELISA法检测70例初诊的RA患者(RA组)和18例健康体检者(健康对照组)血清中IL-22的水平,其中RA组患者进一步分为RA活动组和RA非活动组,有骨侵蚀组和无骨侵蚀组,类风湿因子(RF)阳性组和RF阴性组,抗环瓜氨酸肽(抗CCP)抗体阳性组和抗CCP抗体阴性组。分析IL-22在不同组别的表达及其与RA患者临床及炎性指标的相关性。结果 1RA组患者血清IL-22水平[(80.30±37.73)pg/mL]较健康对照组[(29.29±7.38)pg/mL]明显升高,差异有高度统计学意义(P<0.01);其中RA活动组[(86.58±41.21)pg/mL]与RA非活动组[(63.24±17.98)pg/mL]及健康对照组[(29.29±7.38)pg/mL]比较,差异均有统计学意义(P<0.05);RA组患者有骨侵蚀组[(94.71±42.20)pg/mL]与RA无骨侵蚀组[(56.03±18.16)pg/mL]比较,差异有统计学意义(P<0.05);2IL-22在RF阳性组、抗CCP抗体阳性组[(84.65±38.47)、(100.3±43.68)pg/mL]明显高于RF阴性组及抗CCP抗体阴性组[(59.48±39.01)、(64.12±18.32)pg/mL],差异均有统计学意义(P<0.05);3RA组患者血清IL-22中水平与DAS28、RF呈正相关性(r=0.2883,P=0.0382;r=0.3670,P=0.0074)。结论 RA患者血清IL-22水平明显升高,可能参与了RA的发病机制,可作为RA病情活动及骨侵蚀的预测指标。

The expression and clinical significance of peripheral blood interleukin -22 in patients with rheumatoid arthritis

Objective To test the serum level of IL-22 in patients with rheumatoid arthritis（RA） and analyze its clinical significance. Methods Concentrations of IL-22 in the serum of 70 newly diagnosed RA patients （RA group） and 18 healthy controls （healthy control group） were measured by enzyme-linked immunosorbent assay. Patients of RA group were further divided into active RA group and non-active RA group, bone erosions group and without bone erosions group, rheumatoid factor （RF） positivity group and RF negative group, anti-cyclic citrullinated peptide antibodies （anti-CCP） positive group and anti-CCP negative group. The expression of IL-22 in different groups and the correlation be-tween IL-22 and the clinical inflammatory indexes of RA patients were analyzed. Results ①Serum IL-22 content in RA group [（80.30±37.73） pg/mL] were significantly higher than that of healthy control group [（29.29±7.38） pg/mL], the difference was statistically significant （P〈 0.01）. There was a significant difference between active RA group [（86.58±41.21） pg/mL], non-active RA group [（80.30±37.73） pg/mL] and healthy control group [（29.29±7.38） pg/mL], the differ-ences were statistically significant （P〈 0.05）. There was a significant difference between bone erosions group [（94.71±42.20） pg/mL] and without bone erosions group [（56.03±18.16） pg/mL] （P〈0.05）;②IL-22 in the RF positive group and anti-CCP antibody positive group [（84.65±38.47）, （100.3±43.68） pg/mL] were higher than those of RF negative groups and anti-CCP antibody negative group [（59.48±39.01）, （64.12±18.32） pg/mL], the differences were statistically significant （P〈0.05）;③the levels of serum IL-22 in RA patients were positive related with disease activity index DAS28 and RF （r=0.2883, P=0.0382;r=0.3670, P=0.0074）. Conclusion The significantly elevated IL-22 in the serum of patients with es-tablished RA indicates that IL-22 may be involved in the pathogenesis of RA, and can be