血管紧张素原基因多态与原发性高血压合并脑梗死的相关性

目的：分析中国汉族人群血管紧张素原（AGT）基因M235T多态与原发性高血压合并脑梗死的关系。方法：应用PCR-直接测序法在150例单纯原发性高血压、135例原发性高血压合并脑梗死及150例健康对照者中，对M235T多态进行基因分型和统计分析。结果：单纯原发性高血压组与健康对照组相比，M235T多态的基因型和等位基因分布无显著差异。原发性高血压合并脑梗死组的基因型分布（TT=8，CT=50，CC=77）分别与单纯原发性高血压组（TT=19，CT=65，CC=66，X^2=6．513，P=0．039）和健康对照组（TT=26，CT=60，CC=64，X^2=10．878，P=0．004）相比，均有显著差异。原发性高血压合并脑梗死组的C等位基因频率显著高于健康对照组（0．756 vs 0．627，X^2=10．992，P=0．001）和单纯原发性高血压组（0．756 vs 0．657，X^2=6．662，P=0．010）。结论：中国汉族人群中，AGT基因M235T多态可能是原发性高血压合并脑梗死发病的遗传危险因素。

The Association Study of Angiotensinogen Gene Polymorphism and Essential Hypertension Complicated by Brain Infarction

Objective: To identify the association of M235T polymorphism of angiotensinogen (AGT) gene with essential hypertension complicated by brain infarction in Chinese Han population. Methods: The genotype was determined by PCR-sequencing in a total number of 285 unrelated hypertensive patients including 135 with brain infarction as well as 150 healthy controls. Results: No statistically association was found between M235T polymorphism and essential hypertension. However, significant differences of genotype distributions were observed in the essential hypertension complicated by brain infarction group(TT=8, CT=50,CC=77) compared with the hypertensive patients (TT=19, CT=65,CC=66, x2 =6. 513, P = 0. 039) and the healthy controls (TT = 26, CT = 60,CC = 64, x2 = 10. 878, P = 0. 004). The essential hypertension complicated by brain infarction patients had a significantly increased C allele frequency than the normal references (0.756 vs 0.627, x2= 10.992, P=0.001) and the hypertensive subjects (0. 756 vs o. 657, x2 =6.662, P=0.010). Conclusion: M235T polymorphism of AGT gene might be a genetic risk factor for essential hypertension complicated by brain infarction in Chinese Han populatioa