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| 几种生药的提取部位对成骨样细胞的增殖作用 | |
| 引用本文： | 殷军,王大为,李发美,陈英杰,姜志明. 几种生药的提取部位对成骨样细胞的增殖作用[J]. 沈阳药科大学学报, 2001, 18(4): 279-282 |
| 作者姓名： | 殷军 王大为 李发美 陈英杰 姜志明 |
| 作者单位： | 1. 沈阳药科大学中药学院 2. 沈阳药科大学药学院 3. 沈阳军区总医院 |
| 摘 要： | 考察了石斛、骨碎补、地骨皮、槲寄生的不同提取部位对成骨样细胞UMR10 6的增殖作用。将 4种生药的水提液、乙醇提取液及其经树脂柱洗脱后的不同提取部位与UMR10 6成骨样细胞共同体外培养 ,用MTT法检测各部位对细胞的增殖作用。结果表明 :除石斛的活性部位在 6 0 %、90 %的醇层外 ,骨碎补、地骨皮、槲寄生的活性部位集中在水层、30 %的醇层。 |
| 关 键 词： | 石斛 骨碎补 地骨皮 槲寄生 细胞的增殖作用 成骨样细胞UMR106 MTT法 |
| 文章编号： | 1006-2858(2001)04-0279-04 |
| 修稿时间： | 2000-11-13 |
The effect of the extracts of some crude drugs on proliferation of osteoblast-like UMR 106 cells |
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| YIN Jun,WANG Da wei,LI Fa mei,CHEN Ying jie,JIANG Zhi ming. The effect of the extracts of some crude drugs on proliferation of osteoblast-like UMR 106 cells[J]. Journal of Shenyang Pharmaceutical University, 2001, 18(4): 279-282 | |
| Authors: | YIN Jun WANG Da wei LI Fa mei CHEN Ying jie JIANG Zhi ming |
| Abstract: | The effects of the extracts of 4 kinds of crude drugs on osteoblast like UMR106 cells were studied. The extracts were incubated with cells in vitro .The proliferation of osteoblast like cells was determined by the MTT assay. The 60% and 90% ethanol extracts of Herba Dendrobii, the water and the 30% ethanol extracts of Rhizoma Drynariae and Herba Visci and Cortex Lycii showed the stimulation activity to osteoblast like cells. |
| Keywords: | Herba Dendrobii Rhizoma Drynariae Herba Visci Cortex Lycii proliferation osteoblast like UMR106 cells MTT assay |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |