首页 | 本学科首页   官方微博 | 高级检索  
     

选择性阻断异常Wnt信号通路对肝癌细胞生长及侵袭的影响
引用本文:江颖,周信达,刘银坤,黄晓武,孙瑞霞,张春晖,陆维祺,朱隽,罗文杰,张华. 选择性阻断异常Wnt信号通路对肝癌细胞生长及侵袭的影响[J]. 复旦学报(医学版), 2010, 37(2): 146-152. DOI:  
作者姓名:江颖  周信达  刘银坤  黄晓武  孙瑞霞  张春晖  陆维祺  朱隽  罗文杰  张华
作者单位:复旦大学附属中山医院普外科,上海200032;江苏省南通大学附属医院普外科,南通226001;复旦大学肝癌研究所,上海200032
摘    要: 目的 构建T细胞转录因子(Tcf)反义RNA真核表达载体以阻断异常Wnt信号通路,探讨其对肝癌细胞生物学特性的影响。方法 利用GeneJammer将反义基因转染人肝癌细胞系SMMC-7721,应用RT-PCR及Western blot方法分别检测转染前后细胞mRNA及蛋白表达差异,通过生长曲线、Transwell小室实验比较转染细胞生长增殖及运动侵袭能力,并进一步用流式细胞仪检测细胞凋亡及生长周期的改变。结果 反义RNA转染降低了Tcf表达,减慢了肝癌细胞的生长速度并抑止其运动侵袭能力。转染反义RNA的肝癌细胞7721-pTas凋亡比例增加[(26.34±2.07)%],明显高于空载体转染细胞7721-vector[(6.53±1.02)%]和亲本SMMC-7721细胞[(4.33±0.68)%] (P<0.001)。同时,处于G0-G1期的反义转染细胞比相应亲本SMMC-7721细胞和转染空载体的细胞7721-vector分别高20.24%和20.95%,而S期细胞比亲本细胞SMMC-7721和7721-vector细胞分别低11.8%和11.38%。结论 反义Tcf RNA转染可通过诱导细胞凋亡和阻止细胞周期的进程而抑制肝癌细胞的恶性增殖,提示选择性阻断异常Wnt信号通路有望成为肝癌基因治疗的新途径。

关 键 词:T淋巴细胞  肝细胞癌  反义核糖核酸  转染
收稿时间:2009-07-06

Selective blockage of abnormal Wnt pathway inhibits the growth and invasion of hepatocarcinoma cells
JIANG Ying,ZHOU Xin-da,LIU Yin-kun,HUANG Xiao-wu,SUN Rui-xia,ZHANG Chun-hui,LU Wei-qi,ZHU Jun,LUO Wen-jie,ZHANG Hua. Selective blockage of abnormal Wnt pathway inhibits the growth and invasion of hepatocarcinoma cells[J]. Fudan University Journal of Medical Sciences, 2010, 37(2): 146-152. DOI:  
Authors:JIANG Ying  ZHOU Xin-da  LIU Yin-kun  HUANG Xiao-wu  SUN Rui-xia  ZHANG Chun-hui  LU Wei-qi  ZHU Jun  LUO Wen-jie  ZHANG Hua
Affiliation:Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China; Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China; Liver Cancer Institute, Fudan University, Shanghai 200032, China
Abstract:Objective To construct the recombinant expression vector encoding antisense Tcf fragment for the blockage of abnormal Wnt pathway, and to investigate its effect on the biological behaviors of human hepatocarcinoma cells. Methods Antisense expression vector was transfected into hepatocarcinoma cells SMMC-7721 with GeneJammer. RT-PCR and Western blot were used to detect Tcf expression. Cell proliferation and motility were compared by growth curves and Transwell plate assay. Cell apoptosis was determined by An...
Keywords:T-lymphocytes  hepatocellular carcinoma  antisense RNA  transfection
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《复旦学报(医学版)》浏览原始摘要信息
点击此处可从《复旦学报(医学版)》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号