Assignment of a human gene for tryptophanyl-tRNA synthetase to chromosome 14 using human-mouse somatic cell hybrids |
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| Authors: | Nobuyoshi Shimizu Raju S. Kucherlapati Frank H. Ruddle |
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| Affiliation: | (1) Department of Biology, Yale University, Kline Biology Tower, 06520 New Haven, Connecticut;(2) Moffett Laboratories, Department of Biochemical Sciences, Princeton University, 08546 Princeton, New Jersey |
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| Abstract: | ![]()
Human tryptophanyl-tRNA synthetase (Trp-RS, EC 6.1.1.2) can be separated from its mouse counterpart by Cellogel electrophoresis. Analysis of the presence or absence of human Trp-RS and other human enzyme markers in eleven independently derived cell lines of human-mouse somatic cell hybrids revealed that the expression of Trp-RS is correlated with the expression of human nucleoside phosphorylase (NP, EC 2.4.2.1). The syntenic relationship between Trp-RS and NP permits the assignment of the structural gene for Trp-RS to human chromosome 14. Karyotype and isozyme analysis of these hybrid clones rules out other linkage assignments. |
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