血凝抑制试验用于肾综合征出血热病人血清分型的研究

在HFRS病毒血清分型的研究中,我们用血凝抑制试验方法成功地将HFRS病毒分为两个血清型,家鼠型和野鼠型。用此法能准确区分HFRS病人的血清型。共检测260例住院病人血清血凝抑制抗体,其中52例为褐家鼠疫区病人血清,可分率达98.39%。208例黑线姬鼠感染的病人血清,可分率达100%。170份血清编码双盲考核,野鼠型HFRS病人符合率达96.24%。家鼠型达83.78%。

Studies on Renal Syndrome Hemorrhagic Fever Sero-types: I. An Identification of Sero-types of HFRS by Hemagglutination Inhibition Assay

The trials of differentiating serotypes of HFRS patients to detect the source of viral infection by neutralization test had been shown to be fail in China. However, this aim can be achieved by hemagglutination inhibition assay (HIA) as shown in this paper. It was found that different titer could be detect among those patients suffering from HFRS of R.norvegicus (R-H) origin or A.agrarius (A-H) origin. The latter (A-H) gave 4-8 times higher titer than the former (R-H). On the other hand, the HIA can detect little difference using immune sera. It is obvious that in China the HFRS infection among patients can be classified into two serotypes by HIA.