胃癌组织中VEGF—C、VGFR-3表达和微淋巴管密度的研究

目的探讨血管内皮生长因子C（VEGF—C）及其受体-3（VEGFR-3）在胃癌组织中的表达，分析其表达与微淋巴管密度（LVD）和胃癌生物学行为之间的关系。方法采用组织微阵列技术，通过免疫组化sP法检测胃癌组织标本125例、癌旁正常组织96例中VEGF—C、VEGFR-3的表达，以Podoplanin标记微淋巴管密度（LVD）进行计数。结果VEGF—C和VEGFR-3在胃癌组织和癌旁正常组织中的阳性表达率分别为62．4％、56．0％和10．41％、12．5％，差异有统计学意义（均P〈0．05），VEGF—C和VEGFR一3表达与LDV、胃癌淋巴结转移、临床分期、远处转移及CEA均呈正相关（P〈0．05），VEGFR一3表达与Ca19—9呈正相关（P〈0．05）而VEGF—C的表达则与CA19—9无相关（P〉0．05）；胃癌组织中LVD计数为（2．98±0．81）个高于癌旁正常组织中计数（1．82±0．63）个，差异有统计学意义（P〈0．05），且癌周LVD计数高于癌内LVD计数（P〈0．05）。结论胃癌组织中VEGF—C和VEGFR-3均呈高表达，与淋巴管生成有关并参与了胃癌的发生、浸润和转移。

A study on expression of VEGF - C and VEGFR - 3 and study on the lymphatic microvessel density in gastric carcinoma

Objective To investigate the expression of the vascular endothelial growth factor C （VEGF - C） and vascular endo- thelial growth factor receptor 3 （ VEGFR - 3） in gastric carcinoma to analyze its relationship between the lymphatic micro vessel density （LVD） and the biological behavior features in the gastric cancer. Methods Applying tissue microarray technology, the immunohistochemical technique SP was used to detect the expression of VEGF - C, and VEGFR - 3 protein in 125 cases of gastric carcinoma and in 96 cases with adjacent normal tissue. The lymphatic vessel density （LVD） was assayed by Podoplanin marks. Results The positive rates of VEGF - C and VEGFR - 3 in gastric cancer tissues were 62.4% and 56.0% ,which were higher than those in adjacent normal tissue expression （ P 〈 0.05 ）. The positive expression rates of VEGF - C and VEGFR - 3 were significantly correlated with LVD, depth of invasion, lymph node metastasis, distant metastasis, clinical stage and CEA in gastrointestinal carcinoma patients （ P 〈 0. 05 ）. The expression of VEGFR - 3 was positively correlated with CA199, but the expression of VEGF - C was not correlated with （ P 〉 0. 05 ）. The LVD score in gastric cancer was significantly higher than that in adjacent normal tissues （2.98 ± 0.81 vs. 1.82± 0. 63 ） （P 〈 0.05 ）, and the peri - tumoral LVD score was higher than intra - tumoral LVD score （P 〈 0.05）. Conclusion The over expression of VEGF - C and VEGFR -3 in gastric cancer can be used to predict lymphangiogenesis of gastric cancer and the invasion process.