脂肪酸、二磷酸果糖和谷氨酰胺对大鼠移植小肠粘膜细胞增殖和凋亡的作用

目的探讨ｎ３脂肪酸、１,６二磷酸果糖和谷氨酰胺对移植小肠粘膜细胞增殖和凋亡的作用。方法１９６只近交系Ｗｉｓｔａｒ大鼠分别作为供、受体行全小肠异位移植,术前和术后分别用ｎ３脂肪酸灌胃、１,６二磷酸果糖及谷氨酰胺静脉输注１０天,应用流式细胞术和细胞凋亡原位检测的方法分析小肠粘膜细胞增殖和凋亡的变化。结果移植小肠粘膜细胞增殖低下,凋亡增加。补充外源性ｎ３脂肪酸、１,６二磷酸果糖和谷氨酰胺后,移植小肠粘膜细胞的增殖加速,凋亡减少。结论ｎ３脂肪酸、１,６二磷酸果糖和谷氨酰胺特殊营养支持可显著地增加移植小肠粘膜细胞的增殖活性,同时也不同程度地抑制细胞凋亡发生,这种调控作用有助于改善移植小肠的结构和吸收功能。

The effects of n-3 fatty acid, fructose-1, 6-diphosphate and glutamine on mucosal cell proliferation and apoptosis of small bowel graft after transplantation in rats

Objective To evaluate the effects of n3fatty acid (n3 FA),fructose1, 6diphosphate (FDP) and glutamine (GLN) on mucosal cell proliferation and apoptosis of small bowel graft.5HZMethods 196 inbred strain Wistar rats underwent heterotopic small bowel transplantation (SBT) as donors and recipients respectively. n3FA,FDP and GLN were given via gastrolavage as well as venous infusion within total parenteral nutrition (TPN) solution for 10 days before and after surgery. Flow cytometry and in situ cell death detection were used for analysing the changes of mucosal cell proliferation and apoptosis. Results Fewer proliferation and more apoptosis took place in mucosal cells of small bowel graft after transplantation.A higher mucosal cell proliferative index and lower apoptotic index were found in all small bowel graft after supplying with n3FA, FDP and GLN. Conclusionsn3FA,FDP and GLN specific nutritional support significantly promoted mucosal cell proliferation, and on the other hand inhibited mucosal cell from undergoing apoptosis with different degrees. The effects of regulation help improve the structure and absorption function of transplanted small bowel.