基于流式细胞术诊断结核感染的研究进展

结核病是危害人类健康的一种传染病,快速诊断与合理治疗是控制结核病的关键,然而对于潜伏性结核感染仍缺少诊断的金标准。目前,结核菌素皮试以及IFN-γ释放试验被视为诊断是否感染结核的有效方法,它们通过间接检测受试者对结核分枝杆菌的细胞免疫反应做出评判,但这两种方法均存在缺陷,也无法明确区分潜伏性感染和活动性结核。流式细胞术作为一项高新技术已经在医学临床领域得到广泛应用。近年来,流式细胞术检测胞内细胞因子技术在结核感染的诊断及疗效评价等方面取得了一定进展。本文对应用流式细胞术进行结核感染诊断的进展进行综述。

Tuberculosis diagnosis based on Flow Cytometry

Tuberculosis (TB) is an infectious disease endangering people’s health around the world, and diagnosis and rational treatment are the key to control TB. However, currently there is no diagnostic golden standard for latent tuberculosis infection (LTBI). The tuberculin skin test and IFN-gamma release analysis are regarded as effective methods for TB diagnosis, which is based on host’s cellular immune response to mycobacterial antigens. But the two methods are not perfect; neither test can make a clear distinction between latent infection and active TB. Flow Cytometry (FCM) has been widely used in the field of clinical medicine. In recent years, substantial advances have been achieved in the fields of TB diagnosis and evaluation of treatment efficacy by using the FCM intracellular cytokine detection technology. In this paper, we will review the latest progress in tuberculosis diagnosis research by using flow cytometry in recent years.