贵州省一例人感染猪链球菌病例的病原学检测与分析

目的 对贵州省一疑似人感染猪链球菌病患者的血液来源菌株进行鉴定。方法 将患者血液分离菌株接种羊血琼脂平板,经革兰氏染色镜检、血清凝集试验和生化反应后,再用PCR技术检测猪链球菌种特异性基因(16S rRNA)和猪链球菌2型特有的荚膜多糖编码基因(cps2J)以及溶菌酶释放相关蛋白编码基因(mrp)、溶血素基因(sly)和细胞外蛋白因子编码基因(ef)。结果 传统方法和PCR技术将病例来源菌株鉴定为猪链球菌2型,PCR鉴定结果显示该菌株的毒力基因cps2J、mrp、sly和Ef均为阳性。结论 贵州省一例人感染猪链球菌疑似病例来源菌株为猪链球菌2型强毒株。

Etiologic detection and analysis of a human Streptococcus suis infection in Guizhou Province,China

In this study, we identified bacteria strain from the blood sample of a patient in a suspected epidemic of human Streptococcus suis infections, investigated the etiologic characteristics and provided etiologic basis for epidemic determination and the diagnosis and treatment of the patient. Suspicious bacteria strain of S. suis from the blood sample of a patient in a suspected epidemic of Streptococcus suis infections was identified using gram staining, serum agglutination test and biochemical test. Streptococcus suis species specific gene 16S rRNA, Streptococcus suis serotype 2 specific virulence gene capsule polysaccharide 2J (cps2J) and virulence gene muramidase-released protein (mrp), hemolysin gene (sly) and extracellular factor protein gene (ef) were further detected by PCR. The suspicious bacteria strain of S. suis was identified as Streptococcus suis type 2 by conventional methods and PCR. PCR detection results showed that 16S rRNA, cps2J, mrp, sly and ef genes of S. suis were positive. This epidemic was caused by human infection with virulent S. suis type 2, which provided etiologic basis for the epidemic determination and the diagnosis and treatment of the patient.