牛磺酸对半乳糖性白内障大鼠房水和晶状体Ca2+含量及晶状体Ca2+-ATP酶活性的影响

目的探讨牛磺酸对半乳糖性白内障房水和晶状体中Ｃａ２＋含量及晶状体Ｃａ２＋－ＡＴＰ酶活性的影响。方法３３只２２ｄ龄Ｗｉｓｔａｒ大鼠随机分为正常对照组、白内障组和实验组；用Ｄ－半乳糖诱发半乳糖性白内障后，实验组在实验的ｄ４结膜下注射１５０ｇ·Ｌ－１牛磺酸；于实验的ｄ８、ｄ１４取房水和晶状体进行各项指标测定。结果白内障组与正常对照组相比，房水和晶状体中Ｃａ２＋含量明显升高，而晶状体Ｃａ２＋－ＡＴＰ酶活性下降；实验组与白内障组相比，房水和晶状体中Ｃａ２＋含量明显降低，而晶状体Ｃａ２＋－ＡＴＰ酶活性恢复至接近正常。结论牛磺酸能够抑制半乳糖性白内障发生过程中晶状体和房水Ｃａ２＋含量的增高并能提高晶状体囊膜Ｃａ２＋－ＡＴＰ酶活性。

Effect of taurine on the quantity of Ca2 and the activity of Ca2 ATPase in galactose cataract mice's aqueons humor and lens

Objective To Probe the effect of taurine on the quantity of Ca2 and the activity of Ca2 ATPase in galactose cataract mice's aqueous humor and lens. Method Mouse were divided into three groups:the normal,the cataract and the experimental group.After induced galactose cataract by Dgalactose,the experimental mouse were subconjunctivally injected 15gL-1 taurine from the 4th day.At the 8th,14th day of the experiment,the quantity of Ca2 and the activity of Ca2 ATPase were measured. Results The quantity of Ca2 in galactose cataract mouse's aqueous humor and lens rised,but the activity of Ca2 ATPase lowed compared with the normal control group.By taurine injection,the quantity of Ca2 lowed in aqueous humor and lens,and the activity of Ca2 ATPase rised to almost normal level. Conclusion Taurine can restrain the rise of quantity of Ca2 in galactose cataract mice's aqueous humor and lens,and recover the activity of Ca2 ATPase of galactose cataract mice's lens.