缺血性心脏病与β-纤维蛋白原基因bcl-I多态性和血浆纤维蛋白原水平的关系

目的:探讨β纤维蛋白原基因bcl-I多态性与血浆纤维蛋白原水平及缺血性心脏病发病机制的关系。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对57例心肌梗塞组和50例健康对照组进行β-纤维蛋白原基因bcl-I多态性分析;采用组织凝血活酶法测定血浆纤维蛋白原水平。结果:酶切后可见在2 500 bp和1 100 bp+1 400 bp碱基对位置分别发现2个等位基因B1和B2。携带B1等位基因心肌梗塞患者血浆纤维蛋白原水平(3.28±0.94)g.L-1明显高于健康对照组(2.74±0.54)g.L-1(P<0.05),携带B2等位基因心肌梗塞患者血浆纤维蛋白原水平(3.97±1.02)g.L-1明显高于健康对照组(3.25±0.61)g.L-1(P<0.05);B2等位基因频率明显增高(P<0.05);B2基因携带者血浆纤维蛋白原水平明显高于同组内B1基因携带者血浆纤维蛋白原水平(P<0.05)。结论:血浆纤维蛋白原水平升高与心肌梗塞有关联;β-纤维蛋白原基因bcl-I多态性与血浆纤维蛋白原水平及心肌梗塞有关联,β-纤维蛋白原基因bcl-I多态性可能是缺血性心脏病危险因素及遗传易感标志之一。

Association of β-fibrinogen bcl-I gene polymorphism and plasma fibrinogen level with ischemic heart disease

Objective To research the association of β-fibrinogen bcl-I polymorphisms and plasma fibrinogen level with the pathogenesis of ischemic heart disease.Methods 57 patients with myocardical infarction and 50 healthy controls were enrolled in this trail.The polymorphisms of β-fibrinogen bcl-I gene was detected by polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP).Plasma fibrinogen levels were assayed by tissue thromboplastin.Results Two allele,B1 and B2,were found at 2 500 bp and 1 100 bp+1 400 bp,respectively.The plasma fibrinogen level,(3.28±0.94)g·L~(-1),in patients with allele B1 was significantly higher than that in the healthy control subjects,(2.74±0.54)g·L~(-1)(P<0.05),as well as with allele B2(3.97±1.02)g·L~(-1) vs(3.25±0.61)g·L~(-1). The plasma fibrinogen level in the samples of genotype B2 was higher than that in samples of B1(P<0.05).The frequency of genotype B2 allele was higher in cases with myocardical infarction than that in healthy controls(P<0.05).Conclusion There is significant association between the fibrinogen plasma level and myocardical infarction.β-fibrinogen bcl-I gene polymorphism is associated with plasma level of fibrinogen and myocardical infarction.These suggest that β-fibrinogen bcl-I gene polymorphism may be a risk factor and a susceptible predictor of ischemic heart disease.