睾丸摘除后大鼠垂体前叶CGRP免疫反应神经纤维的变化

目的 探讨体内睾酮水平变化对大鼠垂体前叶ＣＧＲＰ免疫反应神经纤维的影响。方法 成年雄性ＳＤ大鼠随机分为７个组；正常对照组（ＮＯＲ），睾丸摘除组（ＯＸ），假手术组（ＳＯ）；正常＝丙酸睾丸素组（ＮＯＲ＋ＴＰ）；睾丸摘除＋丙酸睾丸素组（ＯＴ＋ＴＰ０，正常＋油剂组（ＮＯＲ＋Ｖ）；睾丸摘除＋油剂组（ＯＸ＋Ｖ）。采用免疫组织化学和图像分析方法观察大鼠垂体前叶中ＣＧＲＰ免疫反应神经纤维的变化。

THE CHANGES OF CALCITONIN GENE-RELATED PEPTIDE IMMUNOREACTIVE NERVE FIBERS IN THE ANTERIOR PITUITARY OF THE RAT FOLLOWING ORCHIECTOMY

Objective The present study was aimed at investigating the effects of the changes of testosterone levels on the calcitonin gene related peptide(CGRP) immunoreactive nerve fibers in the anterior pituitary(AP) of the rat.Methods The adult male SD rats were randomly divided into 7 groups: (1)normal(NOR), (2)orchiectomy(OX), (3)shame operation(SO), (4)normal+testosterone propionate(NOR+TP), (5)orchiectomy+testosterone propionate(OX+TP), (6)normal+vehicle(NOR+V), (7)orchiectomy+vehicle(OX+V). Immunohistochemistry and image analysis were used to study the changes of CGRP immunoreactive nerve fibers in the AP of the rat.Results Following orchiectomy 7d,the amount of CGRP immunoreactive nerve fibers in the AP of the rat increased significantly, with the tortuous varicose fibers more densely distributed than that of NOR and the amount of varicosities increased significantly, while the thick fibers were more thicker than that of NOR,and many thin fibers were sent out from the thick fibers, and the varicosities were more larger. Student's t test showed that the difference of the average area density of the CGRP immunoreactive nerve fibers in the AP between OX and NOR was significant. The difference of that between OX+V and NOR was also significant. But the difference of that between SO,NOR+TP,OX+TP,NOR+V and NOR was insignificant, whereas the appearance of the CGRP immunoreactive nerve fibers of NOR+TP and OX+TP was different from that of NOR, with the former two had more thin fibers and less thick fibers.Conclusion The results demonstrated that CGRP immunoreactive nerve fibers in the AP of the rat could respond actively to the violent change of circulating TP, suggesting that CGRP immunoreactive nerve fibers might regulate secretion of the AP gland cells.