补肾调肝清心方对围绝经期抑郁症睡眠障碍模型大鼠行为学的影响

目的观察补肾调肝清心方对围绝经期抑郁症睡眠障碍模型大鼠行为学的影响。方法将60只围绝经期大鼠按体重随机分为5组,每组12只,正常对照（A）组、围绝经期抑郁症睡眠障碍模型（B）组、围绝经期抑郁症睡眠障碍补佳乐治疗（C）组、围绝经期抑郁症睡眠障碍米氮平治疗（D）组、围绝经期抑郁症睡眠障碍中药治疗（E）组。造模后A、B组分别给予生理盐水每日2 mL灌胃;C、D、E组分别予补佳乐（0.117 mg/kg）、米氮平（3 mg/kg）、中药补肾调肝清心方（3.51 mg/kg）灌胃,记录大鼠自主走格次数、自主活动探究次数、糖水偏爱性、强迫游泳不动时间、睡眠潜伏期以及睡眠持续时间。结果自主活动走格次数和探究次数,B组与A组比较差异有统计学意义（P〈0.01）;糖水偏爱性,B组与A组相比较差异有统计学意义（P〈0.01）,C、D组与B组比较差异有统计学意义（P〈0.05）;强迫游泳不动时间,B组与A组比较差异有统计学意义（P〈0.01）,C、D、E组与B组比较差异有统计学意义（P〈0.05,P〈0.01）;睡眠潜伏期,各组间差异均无统计学意义（P〉0.05）;睡眠持续时间,B组与A组比较差异有统计学意义（P〈0.01）,C、D、E组与B组比较差异有统计学意义（P〈0.05,P〈0.01）。结论围绝经期抑郁症睡眠障碍模型大鼠自主走格次数及探究次数、糖水偏爱性、睡眠持续时间均低于正常围绝经期大鼠,强迫游泳不动时间高于正常围绝经期大鼠;补肾调肝清心方可以缩短围绝经期抑郁模型大鼠强迫游泳不动时间以及增加睡眠持续时间。

Effects of Bushen Tiaogan Qingxin Decoction on ethology in perimenopausal depression rats with sleep disorder

Objective To observe the effects of Bushen Tiaogan Qingxin（ BTQ） Decoction on ethology in perimenopausal depression rats with sleep disorder. Methods Sixty perimenopausal rats,according to weight,were randomly divided into a normal group（ A）,a model group（ B）; a progynova treatment group（ C）,a mirtazapine group（ D）,a TCM group（ E）,12 rats in each one. After model establishment,rats in the group A and group B were treated with 2 ml of normal saline every day,while rats in the group C,group D and group E were respectively treated with progynova（ 0. 117 mg / Kg）,mirtazapine（ 3 mg / Kg） and BTQ Decoction（ 3. 51 mg / Kg）.The times of independent walking lattice,times of independent inquiry activity,preference of sucrose,immobility time of forced swimming,sleep latency and duration of sleep were recorded. Methods For the times of independent walking lattice and independent inquiry activity,there was significant difference between group B and group A（ P〈0. 01）. For preference of sucrose,there was significant difference between group B and group A（ P〈0. 01）,and there was significant difference between group C,group D and group B（ P〈0. 01）. For immobility time of forced swimming,there was significant difference between group B and group A（ P〈0. 01）,and there was significant difference between group C,group D,group E and group B（ P〈0. 05,P〈0. 01）. For sleep latency,there was no significant difference among each group（ P〉 0. 05）. For duration of sleep,there was significant difference between group B and group A（ P〈0. 01）,and there was significant difference between group C,group D,group E and group B（ P〈0. 05,P〈0. 01）. Conclusion The times of independent walking lattice,times of independent inquiry activity,preference of sucrose and duration of sleep in the perimenopausal depression rats with sleep disorder were all shorter than those in the normal perimenopausal rats,and immobility time of forced swimming was longer than normal perimeno