PCNA反义寡核苷酸抑制人肝癌细胞体外增殖的研究

目的 观察增殖细胞核抗原（PCNA）反义寡核苷酸对人肝癌细胞株体外增殖的影响及生物学特性的改变，方法 MTT法，集落形成试验观察PCNA反义寡核苷酸对人肝癌细胞的生长抑制，免疫组化技术检测PCNA的蛋白表达，流式细胞仪分析细胞周期变化。结果 PCNA反义核酸作用后的肝癌细胞生长明显受到抑制，抑制效应于作用后48h最为明显，并于72h后逐渐减弱，集落形成率明显下降，PCNA蛋白表达明显下降，细胞周期中S期细胞数明显减少。结论 PCNA反义寡核苷酸能显著抑制人肝癌细胞的生长。

INHIBITION OF HEPATOCELLULAR CARCINOMA CELL PROLIFERATION BY ANTISENSE OLIGONUCLEOTIDES TARGETING THE MESSENGER RNA ENCODING PROLIFERATION CELL NUCLEAR ANTIGEN

Objective To investigate the inhibitory effect of PCNA antisense oligonucleotides on hepatocellular carcinoma cell proliferation.Methods The inhibition of hepatocellular carcinoma cell proliferation was estimated by MTT.The protein expression was analysed by immunohistochemistry S P method.The cell cycle was analysed by flow cytometric(FCM).Results The cell proliferation was inhibited effectively by antisense oligonucleotides.The cell growth rate was reduced nearly 50% after 48 hours treatment.The proportion of S phase was reduced.The inhibit effect discreased after 72 hours.Conclusion PCNA antisense oligonucleotides exerts a strong inhibitory effect on hepatocellular carcinoma cell proliferation.