端粒酶活性在兔后囊膜混浊晶状体上皮细胞中的表达及意义

目的 探讨端粒酶活性在兔后囊膜混浊晶状体上皮细胞中的表达及意义.方法 对新西兰大白兔11只(22只眼)行超声乳化晶状体吸除术法,术后3个月所有实验动物的晶状体后囊膜均已混浊.20只眼分别取赤道部囊膜、混浊后囊膜组织,采用端粒重复序列扩增-聚丙烯酰胺凝胶电泳法定性和端粒重复序列扩增-酶联免疫吸附定量法检测其晶状体上皮细胞端粒酶活性.两者端粒酶活性比较采用成组设计t检验.结果 以HepG2细胞作为端粒酶检测阳性对照,兔晶状体赤道部囊膜组织、后囊膜均可检测到端粒酶活性,表现为自50 bp开始多个模糊梯度条带状电泳图谱,其吸光度A450-690值分别为0.85±0.23、0.67±0.19,两者比较差异有统计学意义(t=2.526,0.021;P＜0.05).结论 在兔后囊膜混浊晶状体上皮细胞中存在端粒酶活性,其活性较晶状体赤道部囊膜上皮细胞低.

Expression and significance of telomerase activity of lens epithelial cells in posterior capsule opacification of rabbits

Objective To explore the expression of telomerase activity of the lens epithelial cells in posterior capsule opacification of rabbits.Methods Clear corneal tunnel phacoemulsification was performed in both eyes of 11 New Zealand rabbits.After the model of posterior capsule opacification succeed in all eyes.Then,telomerase activity of the lens epithelial cells in the equator and posterior capsule opacification of 20 eyes was detected with TRAP-ELISA and TRAP-PAGE techniques.Results HepG2 cells were used as positive controls.Telomerase activity was detected in the lens epithelial cells in the equator capsule and posterior capsule opacification in rabbits,which showed several dim gradient stripped electrophoresis pattern.A450-690 value of telomerase activity in the equator capsule and posterior capsule opacification was 0.85±0.23 and 0.67±0.19,respectively,indicating statistically significant difference(t=2.526,0.021;P＜0.05).Conclusions Telomerase activity exists in the lens epithelial cells of posterior capsule opacification in rabbits.The telomerase activity in this area is lower than that in the equator capsule.