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Identification of proteins by immunochemical methods after electrophoretic runs on different supports (author's transl)]
Authors:J Groulade  M C Denis  E Aveynier
Affiliation:1. Lboratoire de Biochimie A, C.H.U. de Grenoble, 38700 La TroncheFrance;2. Laboratoire d''Exploration Fonctionnelle Rénale, Hôpital des Sablons, 38 700 La TroncheFrance
Abstract:
The determination of the location of serum proteins after electrophoresis on various supports and after immunoelectrophoresis has been achieved by immunochemical methods with specific antisera. The present study describes the technique, gives details of various identified fractions and presents some figures. The method allows us to study the constitution of the "beta-gamma block" observed on paper and acetate supports after electrophoresis of cirrhotic sera and to superimpose the patterns of apparently distinct fractions on polyacrylamide gel.
Keywords:
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