制白附子70％乙醇提取物的长期毒性试验研究

目的：评价制白附子70％乙醇提取物的长期毒性作用。方法：灌胃大鼠25．0g·kg^-1、50．Og·kg^-1及100．0g·kg^-1制白附子70％乙醇提取物或蒸馏水3月，观察给药期间大鼠的一般情况、活动度、毛色、排泄物等，每周称体重和摄食量，于给药末期和恢复末期各处死50％动物进行系统尸解、生化学、血液学及病理学检查。结果：给药3月大鼠的体重、脏器系数、生化学及血液学指标均有一定波动，部分动物见肝脏、前列腺及心肌组织病理学改变，在恢复期可改善。未见药物对大鼠摄食量及一般行为学的影响。结论：大鼠的体重、脏器系数、生化学、血液学指标均在本室背景数据范围内波动，且无明显剂量一毒性关系，提示上述指标的变化无明显毒理学意义。病理检查所见多为大鼠常见自发病变，故不能确定为药物作用所致。综上，制白附子70％乙醇提取物在3个受试剂量下对大鼠的长期毒性作用不明显．且未观察到蓄积作用。

Study on the Chronic Toxicity （3 months） of 70 % Ethanolic Extract from Processed Rhizoma Typhonii on Rats

Objective： To investigate the potential toxicity of 70% ethanolic extract from processed Rhizoma Typhonii on rats. Methods ： 4 groups of 10 SD rats of each sex were administrated with 25.0 g·kg^-1 , 50.0 g ·kg^-1 , 100. 0 g ·kg^-1 70% ethanolie extract of processed Rhizoma Typhonii or distilled water, once a day, for 3 months. Those rats were sacrificed 50% on the 90th or the 105th day, respectively, with autopsy, blood bio-chemical analysis, hematological analysis and pathological ex- amination. Results： There were some pathological changes in liver, prostate and cardiac muscle of some rats. There was no effect on behavior and food consumption of rats. Conclusion： The changes of weight, organ coefficient, blood bio-chemical a- nalysis and hematological analysis all waved in range of our background data, and had no significant dosage-toxicity relationship. Meanwhile, the pathological changes in liver; prostate and cardiac muscle were common spontaneous lesions on rats. In brief, the dosages of 25.0 g ·kg^-1, 50.0 g ·kg^-1 and 100. 0 g ·kg^-1 showed no significant chronic toxicity and accumulation toxicity on rats in our research.