| 发夹式siRNA对hDNMT1基因表达的抑制作用 |
| |
| 引用本文： | 郭颖,黄庆,府伟灵. 发夹式siRNA对hDNMT1基因表达的抑制作用[J]. 重庆医学, 2004, 33(8): 1141-1142 |
| |
| 作者姓名： | 郭颖 黄庆 府伟灵 |
| |
| 作者单位： | 第三军医大学西南医院检验科,重庆,400038;第三军医大学西南医院检验科,重庆,400038;第三军医大学西南医院检验科,重庆,400038 |
| |
| 基金项目： | 国家自然科学基金 , 第三军医大学校科研和教改项目 |
| |
| 摘 要： | 目的研究发夹式siRNA对hDNMT1基因表达的抑制作用.方法根据hDNMT1全长cDNA序列设计和合成与其互补的发夹式siRNA序列,并以发夹式siRNA序列为模板经体外合成siRNA表达盒(SECs).SECs通过脂质体载体转染结直肠癌细胞株HCT116后,经半定量RT-PCR 法检测hDNMT1 mRNA的表达水平. 结果发夹式siRNA 可以特异性地抑制hDNMT1 基因.结论通过SECs产生的发夹式siRNAs 可以特异性抑制hDNMT1 基因的表达;本研究为通过构建表达发夹式siRNA载体抑制hDNMT1基因表达的研究奠定了基础.
|
| 关 键 词： | hDNMT1基因 发夹式siRNA siRNA表达盒 RNA干扰 |
| 文章编号： | 1671-8348(2004)08-1141-02 |
Inhibition of hDNMT1 gene expression by hairpin siRNA inHCT116 cells |
| |
| GUO Ying,HUANG Qing,FU Wei ling. Inhibition of hDNMT1 gene expression by hairpin siRNA inHCT116 cells[J]. Chongqing Medical Journal, 2004, 33(8): 1141-1142 |
| |
| Authors: | GUO Ying HUANG Qing FU Wei ling |
| |
| Abstract: | Objective To research the hDNMT1 gene expression inhibition of hairpin siRNA.Methods According to the gene sequence of hDNMT1 in the GenBank,we designed hairpin siRNA templates,and generated the siRNA expression cassettees.The SECs were transfected into HCT116 cells using the agent siPO RTTM XP 1.The non transfected cells were taken as a control.Inhibitory effect of siRNAs was detected by semi quantitative RT PCR.Results The hairpin si RNA generated from SECs inhibited hDNMT1 gene expression.Conclusion At the cell level,inhibition of hDNMT1 gene expression by hairpin siRNA gene rated by SECs provides basic research for inhibiting hDNMT1 gene expression study. |
| |
| Keywords: | hDNMT1 gene hairpin siRNAs siRNA expression cassettees RNAi |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
