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| 分泌抗人聚白蛋白单克隆抗体杂交瘤细胞株的建立及其应用 | |
| 引用本文： | 蔡美英,张子康,牟家琬,汪成孝,尹廷渊,李明远,刘玲,赵宗蓉,王道若. 分泌抗人聚白蛋白单克隆抗体杂交瘤细胞株的建立及其应用[J]. 四川大学学报(医学版), 1989, 0(2) |
| 作者姓名： | 蔡美英 张子康 牟家琬 汪成孝 尹廷渊 李明远 刘玲 赵宗蓉 王道若 |
| 作者单位： | 华西医大微生物学与免疫学教研室(蔡美英，张子康，牟家琬，汪成孝，李明远，刘玲，赵宗蓉)，成都军区卫生学校(尹廷渊)，华西医大微生物学与免疫学教研室(王道若) |
| 摘 要： | 作者成功地获得了持续稳定分泌抗人聚白蛋白(PHSA)的单克隆抗体(McAb)细胞株。用~(125)I标记该McAb,采用敏感性高的RIA法对HBsAg阳性和阴性血清中老化的白蛋白分子进行了探测。结果发现PHSA主要存在于HBsAg阳性血清中。此工作为乙型肝炎病毒(HBV)与循环中PHSA结合的可能性提供了实验依据。 |
| 关 键 词： | 单克隆抗体 人聚白蛋白 人聚白蛋白受体 杂交瘤 |
DEVELOPMENT AND APPLICATION OF HYBRIDOMA SECRETING MONOCLONAL ANTIBODY AGAINST POLY-HUMAN SERUM ALBUMIN |
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| Cai Meiying,Zhang Zikang,Mu Jiawan,Wang Chengxiao,Yin Tingyuan,Li Mingyuan,Liu Ling,Zhao Zhongrong,Wang Daoruo. DEVELOPMENT AND APPLICATION OF HYBRIDOMA SECRETING MONOCLONAL ANTIBODY AGAINST POLY-HUMAN SERUM ALBUMIN[J]. Journal of Sichuan University. Medical science edition, 1989, 0(2) | |
| Authors: | Cai Meiying Zhang Zikang Mu Jiawan Wang Chengxiao Yin Tingyuan Li Mingyuan Liu Ling Zhao Zhongrong Wang Daoruo |
| Affiliation: | Cai Meiying;Zhang Zikang;Mu Jiawan;Wang Chengxiao;Yin Tingyuan;Li Mingyuan;Liu Ling;Zhao Zhongrong;Wang Daoruo Department of Microbiology and Immunology |
| Abstract: | Two hybridomas which secrete monoclonalantibody (McAb) against polymerized humanserum albumin (PHSA) were obtainted bythe fusion of SP2/0 myeloma cell with im-mune murine spleen cells. One of the McAbwas identified as mouse IgG_1, the other wasIgM. The titers of these purified McAb was1:16 364 with passive hemagglutinationassay (PHA). After labelling with ~(125)I byehloramine-T method, a solidphase radio-immune assay for detecting the PHSA hasyielded in 21 positive results, out of 126HBsAg positive sera, but 53 HBsAg negativesera were all negative. At present we havenot seen any report of PHSA present inciculation. PHSA may be as a bridge bindreceptor between HBV and hepatocytes andthen initiate infection. The appearance of PHSA in HBsAg posi-tive sera could be the result of the damageof the liver during virus infection. More work should be done for thisexplanation. |
| Keywords: | Monoclonal antibodies Poly-human serum albumin Poly-human serum albumin receptor Hybridoma |
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