粘附分子CD44V6及抑癌基因PTEN的表达与葡萄胎恶变关系的探讨

目的　探讨粘附分子CD4 4V6及抑癌基因PTEN表达量与葡萄胎恶变的相关性。方法　 1994～ 2 0 0 0年 ,采用免疫组织化学染色技术检测 15例正常绒毛及 5 5例葡萄胎组织中CD4 4V6及PTEN表达 ,应用图像分析系统定量分析其表达水平。结果　CD4 4V6在正常绒毛组织中无表达 ,在葡萄胎非恶变组及恶变组中表达水平均明显高于正常绒毛组织 (P <0 0 1)。在恶变的葡萄胎CD4 4V6表达明显高于未恶变的葡萄胎组织 (P <0 0 1) ;PTEN在葡萄胎非恶变组及恶变组中水平明显低于正常绒毛组织 (P <0 0 1)。PTEN表达量在恶变的葡萄胎组织明显低于未恶变的葡萄胎组织 (P <0 0 1)。结论　CD4 4V6及PTEN的表达可作为预测葡萄胎恶变的新指标

Prediction of malignant transformation in hydatidiform mole by determining the expressions of CD44V6 and PTEN gene

Objective To study the relationship between the levels of CD44V6,PTEN and malignant transformation of hydatidiform mole.Methods The expressions of CD44V6 and PTEN in 15 samples of normal chorionic villus and 55 cases of hydatidiform mole were detected by means of immunohistochemical staining and image analysis system for quality and quantity tests.Results The expression of CD44V6 in normal pregnancy villus could not be detected.The levels of CD44V6 expression in non-deteriorative and deteriorative hydatidiform mole were significantly higher than those in the normal controls(P<0.01).The level of CD44V6 expression in deteriorative hydatidiform mole was significantly higher than that in non-deteriorative hydatidiform mole (P<0.01).The levels of PTEN expression in the non-deteriorative and deteriorative hydatidiform mole were significantly lower than that in the control (P<0.01).The level of PTEN expression in deteriorative hydatidiform mole was significantly lower than that in the non-deteriorative hydatidiform mole (P<0.01).Conclusion There is correlation between the levels of CD44V6 and PTEN expressions and malignancy of hydatidiform mole.The expressions of CD44V6 and PTEN might be used as the new markers for prediction of malignant transformation of hydatidiform mole.