PC-1基因表达对前列腺癌细胞迁移能力的影响

背景与目的:PC-1基因在雄激素非依赖和高转移能力的前列腺癌C4-2细胞中高表达,在雄激素依赖及不转移的前列腺癌LNCaP细胞中低表达。本实验旨在研究PC-1对前列腺癌细胞迁移能力的影响。方法:构建PC-1稳定高表达的LNCaP细胞株和反义核酸调低内源性PC-1表达的C4-2细胞株。利用体外迁移系统检测PC-1表达对LNCaP和C4-2细胞迁移运动能力的影响。结果:体外迁移实验表明稳定转染提高PC-1的表达水平并未使LNCaP迁移细胞数增多(P>0.05),而反义核酸降低PC-1表达则使C4-2迁移细胞数降低(P<0.05)。PC-1蛋白水平升高不能提高LNCaP细胞迁移能力,但降低内源性PC-1表达则使C4-2细胞迁移能力明显降低。结论:PC-1可能在前列腺癌细胞侵袭过程中起一定作用。

Effect of PC-1 Gene Expression on Migration Ability of Prostate Cancer Cells

BACKGROUND & OBJECTIVE: PC-1 gene is highly expressed in C4-2 cell line, an androgen-independent and aggressive prostate cancer cell line, but lowly expressed in LNCaP cell line, an androgen-dependent prostate cancer cell line. This study was to assess the contribution of PC-1 gene to migration of prostate cancer cells. METHODS: LNCaP cell line with stable expression of PC-1 gene and C4-2 cell line with antisense nucleotide-down-regulated PC-1 were established. The migration abilities of LNCaP and C4-2 cells were tested using an in vitro transwell invasion assay. RESULTS: Migration cell count of LNCaP cells with up-regulation of PC-1 gene was similar to that of control LNCaP cells(P>0.05); migration cell count was significantly lower in C4-2 cells with down-regulation of endogenous PC-1 gene than in control C4-2 cells (P<0.05). CONCLUSION: PC-1 may be involved in the invasion of prostate cancer cells.