大孔吸附树脂提取泰乐星的研究

用几种典型的大孔吸附树脂对发酵过渡液中泰乐星吸附，筛选出Ｈ－３型树脂为吸附剂。静态实验表明，Ｈ－３型大孔吸附树脂对泰乐星吸附量达１５５。．９ｍｇ／ｇ干树脂，最佳吸附ＰＨ在７．５～８．０，吸附剂投入放量７％～８％（ｗ／Ｖ），吸附时间６为最适宜吸附条件。其吸附等温线服从Ｆｒｅｕｎｄｌｉｃｈ吸曙式。以甲醇－柠檬酸缓冲液体系做洗脱剂，解吸率达８４．６％。选出以Ｄ２９０大孔碱性树脂为脱色剂。动态实验确定吸附

Study on extraction of tylosin with marcroporous adsorption resin

Various kinds of marcroporous adsorption resin were used to extract tylosin from filter liquor. The static experiments showed that H 3 marcroporous adsorption resin had the highest adsorption capacity, 155.9 mg/g dry resin, the optimal pH of adsorption was 7.5~8.0, and the operation conditions of the proportion of the resin 7%~8% (w/v) and the adsorption time 6 hours was optimum. The isotherm equation was according to Freundlich type. The recovery rate of 84.6% could be obtained in application of methanol citric acid buffer as eluent.The marcroporous alkaline resin D 290 was selected to served as decolorant. The dynamic experiments showed when the flow rate of tylosin solution was 2BV/h, and that of eluent was 1BV/h, there was a obvious eluent peak of tylosin. By this method the purity of tylosin could reach 97%.