实验性Budd—Chiari综合征的制作

用自行设计的昆布缩窄器开发供放射学研究的Ｂｕｄｄ—Ｃｈｉａｒｉ综合征（ＢＣＳ）模型。昆布缩窄器由一组四片昆布和一个塑料外环组成。４６只Ｗｉｓｔａｒ鼠分为急性ＢＣＳ组１４只，慢性ＢＣＳ组２０只和对照组１２只。麻醉鼠并暴露其肝上段下腔静脉（ＳＩＶＣ），借助直径不同的纱布卷放置缩窄器于ＳＷＣ周围．术后作静脉造影、ＭＲ成像、门脉压测定和组织病理检查随访。急性组全部死于肝流出道阻塞。慢性组平均门脉压为２．１８ＫＰａ，明显高于对照组１．６４ＫＰａ（Ｐ＜０．０１）。慢性组中ＳＩＶＣ完全闭塞５只鼠（２６．３％），严重狭窄１２只鼠（６３．２％）。急性组和慢性组的病理表现与临床所见类似。结论：用昆布缩窄器能建立可靠的ＢＣＳ鼠模型，可用于放射学在内的广泛实验研究。

Experimental Production of Budd-Chiari Syndrome

PURPOSE: To produce Budd-Chiari syndrome (BCS)model for radiologic studies by using a self-devised Laminaria constrictor. MATERIALS AND METHODS: A Laminaria constrictor consisted of four Laminaria pieces and a plastic outer rim. Forty-six Wistar rats were divided into an acute BCS group (n=14),a chronic group (n= 20), and a control group (n=12). After anesthetization and exposure of the suprahepatic inferior vena cava (SIVC)in rats,a constrictor was placed around the SIVC with an aid of a gauze roll 0. 5cm or 1. 0cm in diameter. Venography, MR image, measurement of portal pressure, and histopathologic studies were performed as follow-up procedures. RESULTS: All acute rats died in severe hepatic out flow block. The portal pressure averaging 2. 181 kPa in the chronic group was significantly higher than that 1. 64 kPa in the control(p<0. 01). Complete occlusion of the SIVC was opacified in five rats (26. 3%),and severe stenosis in 12(63. 2%)in the chronic group. The histopathologic findings of both acute BCS rats and chronic animals are comparable to those observed in human BCS cases. CONCLUSION:The Laminaria constrictor can be applied to produce a reliable BCS model for widespread experimental research including radiologic studies.