| 小鼠Dlx-5 cDNA的克隆及序列测定 |
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| 引用本文： | 屈铁军,吴补领,高杰,逄键梁. 小鼠Dlx-5 cDNA的克隆及序列测定[J]. 临床口腔医学杂志, 2005, 21(1): 8-10 |
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| 作者姓名： | 屈铁军 吴补领 高杰 逄键梁 |
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| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032;第四军医大学口腔医学院,陕西,西安,710032;第四军医大学口腔医学院,陕西,西安,710032;第四军医大学口腔医学院,陕西,西安,710032 |
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| 基金项目： | 全军“十五”计划基金重点课题 (0 1Z0 89) |
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| 摘 要： | 目的 :扩增小鼠Dlx 5的特异性片段 ,构建重组质粒并进行序列测定 ,为进一步的蛋白表达、抗体制备、基因转染等研究奠定基础。方法 :提取E14d小鼠的下颌弓组织总RNA ,以反转录cDNA为模板进行PCR反应 ,克隆Dlx 5至PMD 18T载体上并测序。结果 :从胎鼠下颌弓组织中克隆到Dlx 5基因片段约 869bp ,重组质粒经酶切显示获得正确重组子 ,测序结果与已知序列吻合。结论 :成功地从小鼠下颌弓中克隆到了Dlx 5基因 ,提示胎鼠的下颌弓组织中有Dlx 5基因的表达。
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| 关 键 词： | 小鼠 Dlx-5基因 克隆 |
| 文章编号： | 1003-1634(2005)01-0008-03 |
Cloning and sequencing of the coding region of mouse Dlx-5 gene |
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| QU Tie-jun,WU Bu-ling,GAO jie,PANG Jian-liang.College of Stomatology,The Fourth Military Medical University,Xi'an ,China. Cloning and sequencing of the coding region of mouse Dlx-5 gene[J]. Journal of Clinical Stomatology, 2005, 21(1): 8-10 |
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| Authors: | QU Tie-jun WU Bu-ling GAO jie PANG Jian-liang.College of Stomatology The Fourth Military Medical University Xi'an China |
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| Affiliation: | QU Tie-jun,WU Bu-ling,GAO jie,PANG Jian-liang.College of Stomatology,The Fourth Military Medical University,Xi'an 710032,China |
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| Abstract: | Objective:To clone the complete encoded sequence mouse Dlx-5 gene from mouse tooth germ, sequence and analyze its sequence.Method: Total RNA was isolated from embryo mouse mandible arch of 14 days and reverse-transcribed into cDNA.The desired cDNA was obtained by PCR. The segments was inserted into PMD-18 T vector and the positive clone was sequenced. Result: Dlx-5 cDNA was obtained from embryo mouse mandible arch. The sequence was consistent with that reported. One mutation base was found in this sequence.Conclusion: The Dlx-5 gene was sucessfully amplfied and sequenced. And this study provides the evidence of expression of Dlx-5 in embryo mouse mandible tissue. |
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| Keywords: | mouse Dlx-5 clone |
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