中药消痈溃得康对乙酸胃溃疡模型大鼠TFF-2表达的影响

目的研究消痈溃得康对乙酸法大鼠胃溃疡模型治疗作用的分子机制。方法将大鼠随机分为正常对照组、假手术对照组、胃溃疡模型组、消痈溃得康治疗组及奥美拉唑治疗组。局部注射乙酸法制备胃溃疡模型,术后第2天开始给药,术后第12天取材,取血清,ELISA方法检测血清三叶因子-2(trefoilfactorfamily2,TFF-2)含量。取溃疡局部胃组织,一部分胃组织用Trizol方法提取RNA,RT-PCR法检测胃组织TFF-2mRNA表达,另一部分胃组织匀浆,ELISA方法检测胃组织TFF-2蛋白表达量。结果模型组大鼠胃组织TFF-2mRNA表达水平明显高于正常对照组和假手术对照组(P0.05),模型组大鼠胃组织及血清TFF-2蛋白含量高于正常对照组和假手术对照组,但统计学差异不显著。消痈溃得康治疗组和奥美拉唑治疗组大鼠胃组织TFF-2mRNA和蛋白表达水平及血清TFF-2蛋白含量明显高于胃溃疡模型组(P0.05)。结论消痈溃得康促进胃溃疡愈合作用的分子机制之一可能是与其上调胃组织TFF-2mRNA及蛋白表达有关。

The effect of Xiaoyongkuidekang on the expression of TFF-2 in rat ulcer model

Objective To study the molecular mechanism of Xiaoyongkuidekang treatment on gastric ulceration in rats model. Methods Rats were divided into 5 groups randomly： normal group, sham group, ulcer model group, xiaoyongkuidekang group and omeprazole group. Acetic acid was applied to a certain part of the gastric mucosa to establish the ulcer model. The content of TFF-2 in stomach and serum was measured by ELISA method. A part of the tissue taken from the focal area was used to extract RNA by Trizol method and the expression of TFF-2 mRNA was measured by RT-PCR method. Results The expression of TFF-2 mRNA was much higher in ulcer model group than in control group or in sham group （P0.05）. The TFF-2 content in gastric tissue and serum was higher in ulcer model group than in control group or in sham group but with no statistical difference. The expression of TFF-2 mRNA in gastric tissue and content of TFF-2 protein in gastric tissue and serum were much higher in xiaoyongkuidekang group and the omeprazole group than in ulcer model （P0.05）. Conclusion The molecular mechanism of promoting the healing of gastric ulcer by Xiaoyongkuidekan may be related to the upregulated expression of TFF-2 mRNA and protein.