铁皮石斛中多糖和甘露糖含量测定方法的改进及与齿瓣石斛的比较研究

目的：改进铁皮石斛中多糖和甘露糖的含量测定方法并对铁皮石斛与齿瓣石斛中的多糖和甘露糖含量进行分析比较。方法：采用硫酸-苯酚法测定石斛中多糖的含量；采用高效液相色谱法测定石斛中甘露糖的含量。结果：多糖在2.54～12.87 mg· L-1范围线性关系良好（r＝0.9990），平均回收率为99.02％，RSD为2.6％（n＝6）；甘露糖的平均回收率为101.5％，RSD为1.7％（n＝9）。6批铁皮石斛药材多糖平均含量为45.94％，甘露糖平均含量为32.33％；9批齿瓣石斛药材多糖平均含量为45.12％，甘露糖平均含量为37.88％。结论：所建方法操作比较简单，准确可靠，重现性好，可用于控制铁皮石斛的质量。铁皮石斛与齿瓣石斛多糖含量相当，齿瓣石斛甘露糖含量高于铁皮石斛。

Improvement in Determination for Polysaccharide and Mannose in Dendrobii Officinalis Caulis and Its Comparation with Dendrobii Devoniani Caulis

Objective：To improve the methods for determining polysaccharide and mannose in Dendrobii Officinalis Caulis and com -pare the contents of polysaccharide and mannose in Dendrobii Officinalis Caulis and Dendrobii Devoniani Caulis , respectively.Meth-ods：Sulfuric acid-phenol method was adopted to determine the content of polysaccharide and HPLC was used to determine the content of mannose.Results：The calibration curve of polysaccharide was linear in the range of 2.54-12.87 mg· L-1 （ r=0.9990 ） the aver-age recovery （ n=6 ） of polysaccharide was 99.02% （ RSD =2.6%）.The average recovery （ n =9 ） of mannose was 101.45%（RSD=1.7%）.Conclusion：The methods are convenient , accurate, reliable and reproducible for quality control of Dendrobii Offici-nalis Caulis.Dendrobii Officinalis Caulis and Dendrobii Devoniani Caulis are similar in the content of polysaccharide ; the content of mannose in Dendrobii Devoniani Caulis is higher than Dendrobii Officinalis Caulis .