Bcl-2反义寡核苷酸增强小细胞肺癌细胞化疗敏感性研究

目的研究Bcl-2反义寡核苷酸是否能增强小细胞肺癌细胞NCI-H69对化疗药物的敏感性.方法将NCI-H69细胞培养传代,细胞共分4组,反义寡核苷酸组、正义寡核苷酸组、无义寡核苷酸组和空白对照组,通过脂质体介导的方法将不同寡核苷酸导入细胞中,Western-Blot法检测Bcl-2蛋白的表达.不同浓度的顺铂与阿霉素作用于4组转染后的细胞,MTT法测定细胞存活分数.结果4组细胞中,与空白对照组相比较,反义寡核苷酸组的Bcl-2蛋白表达明显受到抑制,而正义寡核苷酸组和无义寡核苷酸组细胞的Bcl-2蛋白表达则与空白对照组差别不明显.细胞分别同顺铂与阿霉素作用后,反义寡核苷酸组细胞在各个浓度的存活分数均低于空白组,其差异在统计学上有显著性意义(P＜0.01),而转染正义链和无义链组与空白组比较其差异在统计学上无显著性意义.结论Bcl-2反义寡核苷酸能有效封闭Bcl-2基因的表达,增强小细胞肺癌细胞对化疗药物的敏感性.

Study on enhancing chemosensitivity of Bcl-2 antisense oligodexynucleotides on small cell lung cancer

Objective:To study the effects of bcl-2 antisense oligodexynucleotides on chemosensitivity of human small cell lung cancer cell line NCI-H69. Methods:Cultured cells were devided into 4 groups: antisense oligodexynucleotides(ASODN), sense oligodexynucleotides(SODN), nonsense oligodexynucleotides(NSODN) and control. The different bcl-2 oligodexynucleotides was transfected into corresponding cells using Oligofectamine. The expression of bcl-2 was examined by Western-Blot. The cell growth was assessed by MTT test.Results:The bcl-2 expression in ASODN group was significantly inhibited compared to the control group, but it was not obviously inhibited in SODN and NSODN groups. The survival fraction of ASODN group was significantly lower than that of the control group.Conclusion:The bcl-2 ASODN could enhance sensitivity of small cell lung cancer cell to chemotherapy by blocking bcl-2 gene effectively.