Conservation strategy for Pelargonium sidoides DC: Phenolic profile and pharmacological activity of acclimatized plants derived from tissue culture |
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Authors: | Mack Moyo Adeyemi O Aremu Jiri Gruz Michaela Šubrtová Lucie Szü?ová Karel Dole?al Johannes Van Staden |
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Institution: | 1. Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa;2. Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany AS CR, Šlechtitel? 11, 783 71 Olomouc, Czech Republic;3. Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitel? 11, 783 71 Olomouc, Czech Republic |
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Abstract: | Ethnopharmacological relevancePelargonium sidoides DC (Geraniaceae), a popular medicinal plant used in folk medicine in the treatment of respiratory-related infections has gained international prominence due to its usage in several herbal formulations. This has led to high demand and the subsequent decimation of wild populations.Aim of the studyUsing plant tissue culture techniques, Pelargonium sidoides plants were cloned in vitro, acclimatized under greenhouse conditions and evaluated for their phytochemical content and pharmacological activity.MethodsPhenolic content in extracts of in vitro-derived, greenhouse-acclimatized and wild Pelargonium sidoides plants were analyzed using UPLC-MS/MS. The oxygen radical absorbance capacity (ORAC), 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and minimum inhibitory concentration (MIC) of the extracts against bacterial and fungal strains were evaluated.ResultsSimilarities in phenolic profiles were identified confirming the chemical signatures that characterize Pelargonium sidoides plants. Extracts of greenhouse-acclimatized and wild plants exhibited comparable antimicrobial and antioxidant properties.ConclusionsOverall, the study highlights the potential of integrating plant tissue culture technologies in conservation strategies of medicinal plants. In particular, the results strongly suggest the feasibility of both large-scale cultivation and plant part substitution as alternative solutions to the current destructive overharvesting practices of wild Pelargonium sidoides populations. |
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Keywords: | AAPH 2 2&prime -Azobis(2-methylpropionamidine) dihydrochloride ANOVA Analysis of variance DMRT Duncan&rsquo s multiple range test DPPH 2 2&ndash Diphenyl-1-picryl hydrazyl MemTR 6-(-3-Methoxybenzylamino)-9-β-D-ribofuranosylpurine MIC Minimum inhibitory concentration MS Murashige and Skoog medium ORAC Oxygen radical absorbance capacity PGR Plant growth regulator PPF Photon flux density TE Trolox equivalents UPLC Ultra performance liquid chromatography |
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