| 妇血宁荧光分光光度法测定和体外吸收实验研究 |
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| 引用本文： | 高尔,王汝琴,张全胜,高珉,李华洲.妇血宁荧光分光光度法测定和体外吸收实验研究[J].中国生化药物杂志,1997,18(4):197-199. |
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| 作者姓名： | 高尔 王汝琴 张全胜 高珉 李华洲 |
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| 作者单位： | 潍坊医学院天然药物研究所!潍坊，261042，潍坊医学院天然药物研究所!潍坊，261042，济南铁路局卫生处，烟台商业学校医务处，潍坊医学院天然药物研究所!潍坊，261042 |
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| 摘 要： | 妇血宁及其组分具有橙黄色荧光，经荧光光度计扫描，其荧光强度波峰（λf）为450nm，激发光波峰（λex）为380nm。妇血宁浓度与荧光强度之间呈线性关系（r＝0．992）。大鼠离体回肠吸收实验表明，妇血宁可经肠道转运，50min时约吸收10％，其扩散常数为3．2×10－6cm2／s。
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| 关 键 词： | 猪蹄甲 角质多肽 荧光分析 体外吸收实验 |
Fluorescent Spectrophotometry of Fu Xue-Ning and Its Absorption Test in the Rat's Isolated Intentine |
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| Gao Er, Wang Ruqin, Zhang Quansheng, Gao Min, Li Huazhou.Fluorescent Spectrophotometry of Fu Xue-Ning and Its Absorption Test in the Rat''''s Isolated Intentine[J].Chinese Journal of Biochemical Pharmaceutics,1997,18(4):197-199. |
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| Authors: | Gao Er Wang Ruqin Zhang Quansheng Gao Min Li Huazhou |
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| Abstract: | We discovered that Fu Xue-Ning (FXN) and its fractions had fluorescence. Their wave form of fluoreseence were the same, and those wave peak were λf 450 nm and λex 380 nm. It was linear relationship between FXN cocentration and relative fluorescent strenth (r= 0. 992 ). Absorptiontest in the rat' s intestine cofirmed that FXN could be absorbed by 10%, its diffusion coefficient Dwas 3. 2 ×10-6 cm2/sec. |
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| Keywords: | Pig's hoof nail Kretinous polypeptide Fluorescent spectrophotometry Absorption test |
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