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| 鬼针草药材高效液相色谱法指纹图谱的初步研究 | |
| 引用本文： | 张礼菊,胡伟,陈飞虎. 鬼针草药材高效液相色谱法指纹图谱的初步研究[J]. 中国基层医药, 2011, 18(14): 1875-1877. DOI: 10.3760/cma.j.issn.1008.6706.2011.14.002 |
| 作者姓名： | 张礼菊 胡伟 陈飞虎 |
| 作者单位： | 1. 安徽医科大学第三附属医院药剂科,安徽省合肥,230061 2. 安徽医科大学第二附属医院药剂科 3. 安徽医科大学药学院 |
| 基金项目： | 国家重大新药创制专项，安徽省高校省级自然科学研究项目 |
| 摘 要： | 目的 对鬼针草药材高效液相色谱法（HPLC）指纹图谱进行研究,建立鬼针草药材的指纹图谱研究方法.方法 采用HPLC,ODS C18色谱柱（4.6 mm ×255 mm,25μm）;乙腈-3%冰乙酸线性梯度洗脱;流速：1.0 ml/min;检测波长：363 nm.结果 建立了鬼针草药材的标准指纹图谱,并检测10批鬼针草药材与标准指纹图谱的相似度在0.941～0.999之间.结论 本实验所建立的方法具有准确可靠、重复性好、特征性强、方法简便等特点,可作为鬼针草药材的质量评价的重要依据之一. |
| 关 键 词： | 鬼针草属 高效液相色谱 指纹图谱 |
Studies on fingerprint of bidens bipinnata L.by HPLC |
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| ZHANG Li-ju,HU Wei,CHEN Fei-hu. Studies on fingerprint of bidens bipinnata L.by HPLC[J]. Chinese Journal of Primary Medicine and Pharmacy, 2011, 18(14): 1875-1877. DOI: 10.3760/cma.j.issn.1008.6706.2011.14.002 | |
| Authors: | ZHANG Li-ju HU Wei CHEN Fei-hu |
| Affiliation: | .(Department of Pharmacy,The Third Hospital of Anhui Medical University,Hefei,Anhui 230061,China) |
| Abstract: | Objective The fingerprint method based on high-performance liquid chromatography for the quality control of bidens bipinnata L were firstly described and optimized. Methods HPLC system was used to obtain the fingerprint spectrum of bidens bipinnata L. The chromatography condition was ODS C18 column （4. 6mm×255mm,25μm）,mobile phase Methanol-0. 2% HAc,and flow speed 1. 0ml ·min^-1, detection wavelength 363nm.Results In this experiment,the standard fingerprint had been established and the similitude degree of 10 batches bidens bipinnata L. has also been obtained（0.941～0.999）. Conclusion The method was accurate,reliable,good reproducibility, strong characteristic and could be used for the standard of general judgement and the quality estimation about the bidens bipinnata L. |
| Keywords: | Bidens HPLC Fingerprint |
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