| Abstract: | The participation of metal ions between C8 and C9 during the final stages of immune hemolysis could not be verified. EA, EAC1-7, and EAC1-8 all lyse completely when tested with 40 mM or higher concentrations of phenanthroline derivatives, only some of which are metal chelators. Only those phenanthrolines that carry a nitrogen in the No. 1 position and are also uncharged are lytic. 1.7-phenanthroline, not a chelator, lyses EAC1-8 more readily than EA and EAC1-7, suggesting an influence of the C5b-8 complex on this type of lysis. Unlike C9 lysis of EAC 1-8, phenanthroline induced lysis of EAC 1-8 cannot be inhibited by incubation with anti-C8, indicating different binding sites for C9 and phenanthrolines.The phenanthrolines neither protect nor promote hypotonic lysis, as do local anesthetics which are known to perturb primarily the membrane lipid phase. Likewise, sublytic drug concentrations do not modify immune hemolysis. |
