三氧化二砷抑制人鼻咽癌细胞侵袭的体外实验研究

 目的 观察三氧化二砷(As₂O₃)对人鼻咽癌细胞株HNEl-LMPl侵袭、转移的影响。方法 鼻咽癌细胞在含3μmol／L的As₂O₃培养基中培养48小时。然后在无含砷的培养基中继续培养48小时后，收集此时间点贴壁的细胞作为研究对象；用细胞-基质黏附实验、细胞运动实验和肿瘤细胞重组基底膜侵袭实验检测As₂O₃对HNEl-LMPl细胞黏附、运动及侵袭能力的影响；用激光共聚焦的方法检测EB病毒编码的潜伏膜蛋白1(LMPl)的表达情况。结果 肿瘤细胞-基质黏附实验结果显示，经As₂O₃处理的HNEl-LMPl细胞，其黏附能力(平均吸光度为0．524±0．09)低于对照组细胞(平均吸光度为0．665±0．14)，两者相比有差异(P<0．05)。运动实验和肿瘤细胞重组基底膜侵袭结果均显示，经As₂O₃处理后，穿过游离的聚乙烯吡咯烷酮膜(PVP-F)的肿瘤细胞数明显减少(P<0.01)，同时As₂O₃能够下调LMPl的表达。结论As₂O₃具有抗人鼻咽癌细胞株转移的潜力，其机制可能与抑制LMPl的表达相关。

Effect of Arsenic Trioxide on Invasion of Human Nasopharyngeal Carcinoma Cells in Vitro

Abstract :Objective 　The study was designed to investigate the effect of arsenic t rioxide (As₂O₃ ) on metastasis of nasopharyngeal carcinoma cell line HNE1-LMP1. Methods 　HNE1-LMP1 cells were cultured in the media containing 3μmol/ L of As₂O₃ for 48 hours and then recovered in the free drug media for following 48 hours. Af terwards , only the attached cells were collected to analyze its metastasis potential. Reconstituted basement membrane invasive assay was used to evaluate the ability of adhesion , migration and invasion in HNE1-LMP1 cells. Confocal microscopy was performed to observe the expression of latent membrane protein 1 (LMP1) . Results 　As₂O₃ significantly inhibited membrane adhesion and invasion as well as migration of HNE1-LMP1 cells. The expression of LMP1 was also reduced as demonst rated by Confocal microscopy examination. Conclusion 　As₂O₃ was a potential agent inhibiting NPC invasion and metastasis. The mechanism might be associated with the reduction of LMP1 expression.