| 人脑型肌酸激酶（CK—BB）的分离和提纯 |
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| 引用本文： | 陆宗良 孙继良. 人脑型肌酸激酶（CK—BB）的分离和提纯[J]. 中国医学科学院学报, 1991, 13(4): 298-301 |
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| 作者姓名： | 陆宗良 孙继良 |
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| 作者单位： | 中国医学科学院心血管病研究所 北京 |
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| 摘 要： | 肌酸激酶(CK)由B及M两个亚单位组成,分子量83000～85000,包括肌酸激酶MM(CK-MM)、肌酸激酶MB(CK-MB)及肌酸激酶BB(CK-BB)三种同功酶。CK-BB的抗体能与CK-MB发生交叉反应,但不与CK-MM反应。CK-BB在血中含量甚微,难用CK-BB抗体检出。急性心肌梗塞(AMI)时血中CK-MB增多,并可用CK-BB抗体检出,有利于对AMI作出早期诊断。分离提纯CK-BB是临床应用这一技术的基础。现有的CK-BB分离提纯法,尚存在一些缺点。本文报道改良的从人脑分离提纯CK-BB的方法。
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| 关 键 词： | 肌酸激酶 人脑 分离 提纯 |
Isolation and purification of human brain creatine kinase (CK-BB)] |
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| Z Lu. Isolation and purification of human brain creatine kinase (CK-BB)][J]. Acta Academiae Medicinae Sinicae, 1991, 13(4): 298-301 |
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| Authors: | Z Lu |
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| Affiliation: | Institute of Cardiovascular Diseases, Beijing. |
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| Abstract: | A improved method for isolation and purification of CK-BB from human brain is introduced. CK-BB of fraction IV was purified to approximately 306.9 fold as compared with homogenate of human brain. The specific activity was 322.2 IU/mg protein. The recovery of creatine kinase activity was 52.8%. This fraction was analyzed by SDS-PAGE. It showed a single major band with an estimated molecular weight of 43000 when it stained with coomassie blue. Purity of fraction V was much better than fraction IV, but some CK activity was lost. |
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