山茱萸多糖对青霉素致痫幼鼠学习记忆能力及海马组织脑源性神经因子、神经生长因子表达的影响

目的:探讨山茱萸多糖对青霉素致痫幼鼠学习记忆能力及海马组织脑源性神经因子(BDNF)、神经生长因子(NGF)表达的影响。方法:将30只日龄21 d龄幼鼠随机分为生理盐水对照组、青霉素点燃模型组、山茱萸多糖处理组各10只。生理盐水对照组只接受生理盐水灌胃,不造模;山茱萸多糖处理组在造模成功后灌胃0.05 g/mL山茱萸多糖;青霉素点燃模型组造模成功后灌胃等量生理盐水;连续灌胃28 d。采用Morris水迷宫检测各组幼鼠学习记忆能力;RT-PCR法检测海马组织BDNF mRNA、NGF mRNA表达;Western-blot法检测海马组织BDNF与NGF蛋白含量。结果:与生理盐水对照组比较,青霉素点燃模型组幼鼠学习记忆能力明显降低(P<0.01);与模型组比较,山茱萸多糖处理组学习记忆能力明显升高(P<0.01)。与生理盐水对照组比较,青霉素点燃模型组幼鼠BDNF、NGF mRNA和蛋白表达明显降低(P<0.01);与模型组比较,山茱萸多糖处理组BDNF、NGF mRNA和蛋白表达明显升高(P<0.01)。结论:山茱萸多糖具有提高青霉素点燃幼鼠学习记忆能力的作用,其机制可能是通过上调BDNF和NGF基因表达。

Effects of Cornel Officinalis Polysaccharide on Learning and Memory Ability, Expression of Brain Derived Neurotrophic Factor and Nerve Growth Factor in Hippocampaltissue of Immature Rats with Penicillin-Induced Epilepsy

Objective: To probe into the effects of cornel officinalis polysaccharide on learning and memory ability, expression of brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in hippocampaltissue of immature rats with penicillin-induced epilepsy. Methods: Thirty immature rats at 21 d were randomly divided into the normal saline control group, epilepsy model group and cornus officinalis polysaccharide group, with 10 cases in each group. The normal saline control group only received normal saline gavage, without modeling. In the cornus officinalis polysaccharide group, 0.05 g/mL of cornus officinalis polysaccharide was given in intragastric administration after successful modeling. The epilepsy model group was treated with the same amount of normal saline in intragastric administration after successful modeling. All rats were continuously given intragastric administration for 28 d. Morris water maze was used to test the learning and memory ability of immature rats in each group. The expression of BDNF mRNA, NGF mRNA in hippocampal tissue was determined by RT- PCR, and the protein content of BDNF and NGF was detected by Western-blot. Results: Compared with the normal saline control group, the learning and memory ability of immature rats in the epilepsy model group decreased significantly (P<0.01). Compared with the epilepsy model group, the learning and memory ability of immature rats in the cornus officinalis polysaccharide group increased significantly (P<0.01). Compared with the normal saline control group, the expression of BDNF, NGF mRNA and protein in the epilepsy model group decreased significantly (P<0.01). Compared with the epilepsy model group, the expression of BDNF, NGF mRNA and protein in the cornus officinalis polysaccharide group increased significantly (P<0.01). Conclusion: Cornel officinalis polysaccharide can improve the learning and memory ability of immature rats with penicillin-induced epilepsy by increasing the expression of BDNF and NGF in hippocampus.