HPLC法测定地胆草中4,5-二咖啡酰奎宁酸的含量

目的：建立高效液相色谱法测定地胆草中4，5-二咖啡酰奎宁酸含量的方法。方法：色谱柱为AgilentSBC．。柱（250mm×4．6mm，5μm），流动相为乙腈．0．1％磷酸溶液（17：83）；流速为1．0ml·min-1；检测波长为327nm；柱温为30℃，进样量10μl。结果：4，5-二咖啡酰奎宁酸在0．0235—2．3520txg范围内线性关系良好（r=0．9999），平均加样回收率为98．75％，RSD为1．24％（n=6）。结论：所用方法简便、快速、准确，可作为测定地胆草中4，5-二咖啡酰奎宁酸含量的方法。

Determination of 4,5-Dicaffeoyquinic Acid in Elehantopus Scaber Linn by HPLC

Objective： To establish an HPLC method for the determination of 4,5-dicaffeoyquinic acid in Elehantopus scaber Linn. Methods： The separation was carried out on an Agilcnt SB C Is column （250 mm×4.6mm,5μm）. The mobile phase was acetonitrile-0. 1% phosphoric acid （17： 83） and the flow rate was 1.0 ml ·min-1. The detection wavelength was set at 327nm, tbe column temperature was 30℃ and the injection volume was 10 μl. Results： The ealibration curve showed a good linearity over the range of 0.023 5-2. 352 0μg（r = 0. 999 9）. The average recovery was 98, 75% with RSD of 1.24% （ n = 6）. Conclusion ： The method is simple, rapid and accurate, and can be used as a quantitative analysis method for Elehantopus scaber Linn.