Determination of the parent of origin in nine cases of prenatally detected chromosome aberrations found after intracytoplasmic sperm injection

Prenatal cytogenetic analysis of 71 fetuses conceived by intracytoplasmicsperm injection (ICSI) resulted in the detection of nine (12.7%) chromosomeaberrations including two cases of 47,XXY, four cases involving a 45,X cellline and three autosomal trisomies. Molecular analysis of the parentalorigin of the deleted or supernumerary chromosome was performed by usingpolymorphic microsatellite markers. Six cases involving a sex chromosomeabnormality were found to be of paternal origin while the two trisomiccases that could be analysed were of maternal origin. Two cases involvedthe same infertile couple who had two consecutive ICSI pregnanciesterminated because of a chromosome abnormality. The replaced embryos inboth cases originated from a single batch of ICSI fertilized oocytes ofwhich part was used to initiate the first pregnancy and part wascryopreserved and used to initiate the second pregnancy.