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| HNE1细胞株光动力学治疗前后形态学的动态变化 | |
| 引用本文： | 王承龙,赖金平,唐发清,肖健云,田勇泉,邓波,罗均利. HNE1细胞株光动力学治疗前后形态学的动态变化[J]. 中国耳鼻咽喉颅底外科杂志, 2000, 6(1): 32-35,I000 |
| 作者姓名： | 王承龙 赖金平 唐发清 肖健云 田勇泉 邓波 罗均利 |
| 作者单位： | 1. 湖南医科大学附属湘雅医院耳鼻咽喉科研究室 2. 湖南医科大学附属湘雅医院中心实验室 3. 湖南医科大学附属湘雅医院皮肤科，湖南长沙 410008 |
| 基金项目： | 国家自然科学基金资助项目(39800030) |
| 摘 要： | 目的 探讨体外光动力学治疗（ＰＤＴ）引起鼻咽癌（ＮＰＣ）细胞死亡的作用机制。方法对鼻咽癌细胞株ＨＮＥ１体外光动力学治疗前后形态学变化进行动态观察。结果 对照组ＨＮＥ１细胞培养３ｈ，６ｈ，１２ｈ，２４ｈ和４８ｈ后细胞形态与正常培养组ＨＮＥ１细包开始变形皱缩，细胞界限模糊，细胞透光度和立体感较差，治疗２４～４８ｈ后细胞发生凋亡。结论 体外光动力学治疗对ＨＮＥ１具有明显的杀伤效应并ＨＮＥ１细胞凋亡。ＰＤ |
| 关 键 词： | 鼻咽肿瘤 HNE1细胞 光动力学疗法 |
| 文章编号： | 1007-1520(2000)01-0032-04 |
Photodynamic therapy induces morphological changes of HNE1 cells in vitro |
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| WANG Cheng long,LAI Jin ping,TANG Fa qing,et al.. Photodynamic therapy induces morphological changes of HNE1 cells in vitro[J]. Chinese Journal of Otorhinolaryngology-skull Base Surgery, 2000, 6(1): 32-35,I000 | |
| Authors: | WANG Cheng long LAI Jin ping TANG Fa qing et al. |
| Affiliation: | WANG Cheng-long(Department ofOtolaryngology,Xinang Ya Hospital,Hunan Medical University,Changsha 410008,China)LAI Jin-ping(Department ofOtolaryngology,Xinang Ya Hospital,Hunan Medical University,Changsha 410008,China)TANG Fa-qing(Department ofOtolaryngology,Xinang Ya Hospital,Hunan Medical University,Changsha 410008,China) |
| Abstract: | Objective To study the mechanism of photodynamic therapy (PDT) which induces the cell death of nasopharyngeal carcinoma. Methods Both before PDT and at 3h, 6h, 12h, 24h and 48h after PDT, morphological changes of HNE1 in vitro were observed by microscope. Results Characteristic changes of HNE1 apoptosis were significantly observed after PDT, including early occurrence of cell condensation, karyopyknosis and karyorrhesis. Conclusion Apoptosis is involved in the process of HNE1 cell death induced by PDT in vitro. the detailed mechanism controlling the phenomenon need to be elucidated by further studies. |
| Keywords: | NASOPHARYNGEAL NEOPLASMS/ther PHOTODYNAMIC THERAPY TUMOR CELLS CULTURED |
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